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Journal of Fluorescence - published by
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Quinoxaline derivatives are a great interest as fluorescent emitters for peroxyoxalate chemiluminescence. Reaction of peroxyoxalates
such as bis-(2,4,6-trichlorophenyl) oxalate with H2O2 can transfer energy to fluorophore via formation of dioxetanedione intermediate. Two quinoxaline derivatives used as a fluorophore
in this study which produce a green light in the chemiluminescence systems. The relationship between the chemiluminescence
intensity and concentrations of fluorophore, peroxyoxalate, sodium salicylate and hydrogen peroxide was investigated. Kinetic
parameters for the peroxyoxalate-chemiluminescence were also calculated from the computer fitting of the corresponding chemiluminescence
intensity/time profiles. It was found that the biphenylquinoxaline can be used as an efficient green fluorescent emitter.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0601-9
Authors
Abdolraouf Samadi-Maybodi, University of Mazandaran Analytical division, Faculty of Chemistry Babolsar Iran
Reza Akhoondi, University of Mazandaran Analytical division, Faculty of Chemistry Babolsar Iran
Mohammad Javad Chaichi, University of Mazandaran Analytical division, Faculty of Chemistry Babolsar Iran
Spectral characteristics of flutamide drug have been studied in various solvents and β-cyclodextrin (β-CD). The inclusion
complex of flutamide with β-CD is analysed by UV-visible, fluorimetry, FT-IR, 1H NMR, SEM, DSC and AM1 methods. In all solvents,
flutamide exhibits a dual fluorescence. The longer wavelength emission (A band ∼380 nm) is due to intramolecular charge transfer
state (ICT) and the shorter wavelength emission (B band ∼285 nm) originates from a locally excited state. In β-CD, the increase
in the fluorescence intensity of ‘A’ band indicates ICT emission enhanced in the β-CD medium. β-CD studies shows isopropyl
group is present in the interior part of the β-CD cavity whereas amino and CF3 groups are present in the outside of the β-CD cavity. A mechanism is proposed to explain the 1:1 inclusion process.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0623-3
Authors
A. Anton Smith, Annamalai University Department of Pharmacy Annamalai nagar 608 002 Tamilnadu India
R. Manavalan, Annamalai University Department of Pharmacy Annamalai nagar 608 002 Tamilnadu India
K. Kannan, Annamalai University Department of Pharmacy Annamalai nagar 608 002 Tamilnadu India
N. Rajendiran, Annamalai University Department of Chemistry Annamalai nagar 608 002 Tamilnadu India
Formulations for the total fluorescence intensity of fluorescent microspheres in slabs of cardiac tissue were determined experimentally
and theoretically. The tissue depth, at which the slab can be considered as a semi-infinite turbid medium, and critical layer
thickness, which accounts for the most emission intensity were evaluated to be 8–9 and 3–5 mm, respectively, for the cardiac
tissue. When fluorescent microspheres are linearly distributed across the slab depth, the mean absorption of them is proportional
to the sum of their normalized total emissions in the slab excited from both sides. The formulations may be used for the fluorescence
images analysis of cardiac and other biological tissues.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0629-x
Authors
Eugene Gussakovsky, Institute for Biodiagnostics, National Research Council Canada 435 Ellice ave. Winnipeg Manitoba R3B 1Y6 Canada
Yanmin Yang, University of Manitoba Faculty of Medicine Winnipeg Manitoba R3E 3P5 Canada
In this study a new fluorescent Schiff base; 1,1′-(4,4′-oxybis(4,1-phenylene)bis(azan-1-yl-1-ylidene))bis(methan-1-yl-1-ylidene)dinaphthalen-2-ol
(2-HNA) was synthesized and characterized by FT-IR, UV-vis, and 1H and 13C-NMR techniques. Photoluminescent properties of 2-HNA were investigated in different solvents including methanol, THF, DMF,
DMSO, acetone, acetonitrile, and dichloromethane. 2-HNA was found to have higher emission intensity and Stoke’s shift value
(∆λST) in methanol solution. Relative emission intensity changes (I0−I/I0) of 2-HNA in methanol/water mixtures depending on different Cu+2 ion concentrations were determined and a linearized plot was obtained. Possible interference of some other transition metal
ions was also determined. Sensitivity limit of the new sensor was found to be higher than 5 × 10−7 mol/L. 2-HNA has quite high selectivity against Cu+2 ion and, thus, can be used as a new fluorescence Cu+2 ion sensor in practice.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0620-6
Authors
Mehmet Yıldırım, Çanakkale Onsekiz Mart University Faculty of Sciences and Arts, Department of Chemistry, TR-17020 Çanakkale Turkey
İsmet Kaya, Çanakkale Onsekiz Mart University Faculty of Sciences and Arts, Department of Chemistry, TR-17020 Çanakkale Turkey
Aiming at the derivation of a generalized procedure for the straightforward preparation of particles fluorescing in the visible
and near-infrared (NIR) spectral region, different swelling procedures for the loading of the hydrophobic polarity-probe Nile
Red into nano- and micrometer sized polystyrene particles were studied and compared with respect to the optical properties
of the resulting particles. The effect of the amount of incorporated dye on the spectroscopic properties of the particles
was investigated for differently sized beads with different surface chemistries, i.e., non-functionalized, amino-modified
and PEG-grafted surfaces. Moreover, photostability and leaking studies were performed. The main criterion for the optimization
of the dye loading procedures was a high and thermally and photochemically stable fluorescence output of the particles for
the future application of these systems as fluorescent labels.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0632-2
Authors
Thomas Behnke, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
Christian Würth, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
Katrin Hoffmann, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
Martin Hübner, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
Ulrich Panne, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
Ute Resch-Genger, BAM Federal Institute for Materials Research and Testing Richard-Willstaetter-Str. 11 12489 Berlin Germany
In this paper, ZnSe nanoparticles, which were modified with mercaptoacetic acid (MAA), worked as novel fluorescence sensors
for the quantitative determination of copper(II) and nickel(II). Under the optimal conditions, the fluorescence intensities
of functionalized ZnSe nanoparticles were quenched by the addtion of copper(II) or nickel(II) ions, there were linear relationships
between the relative fluorescence intensity (logF0/F) and the concentration in the range of 140–2,000 μg/L for copper(II) (R = 0.9973) and 30–1,000 μg/L for nickel(II) (R = 0.9992), the limits of detection were 50 μg/L and 5 μg/L, respectively.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0626-0
Authors
Fenfen Zhang, Shanghai University Department of chemistry Shanghai 200444 People’s Republic of China
Li Li, Shanghai University Department of chemistry Shanghai 200444 People’s Republic of China
Yaping Ding, Shanghai University Department of chemistry Shanghai 200444 People’s Republic of China
Yaping Wang, Shanghai University Department of chemistry Shanghai 200444 People’s Republic of China
Aiming at the identification of new fluorescent reporters for targeted optical probes, we assessed the application-relevant
features of a novel asymmetric cyanine, DY-681, in comparison to the only clinically approved dye indocyanine green (ICG),
the golden imaging standard Cy5.5, and the asymmetric cyanine DY-676 successfully exploited by us for the design of different
contrast agents. This comparison included the analysis of the spectroscopic properties of the free fluorophores and their
thermal stability in aqueous solution as well as their cytotoxic potential. In addition, the absorption and emission features
of IgG-conjugated DY-681 were examined. The trimethine DY-681 exhibited spectral features closely resembling that of the pentamethine
Cy5.5. Its high thermal stability in phosphate buffer saline (PBS) solution in conjunction with its low cytotoxicity, reaching
similar values as determined for Cy5.5 and DY-676, renders this dye more attractive as ICG and, due to its improved fluorescence
quantum yield in PBS, also superior to DY-676. Although in PBS, Cy5.5 was still more fluorescent, the fluorescence quantum
yields (Φf) of DY-681 and Cy5.5 in PBS containing 5 mass-% bovine serum albumin (BSA) were comparable. Labeling experiments with DY-681
and the model antibody IgG revealed promisingly high Φf values of the bioconjugated dye.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0603-7
Authors
Jutta Pauli, BAM Federal Institute for Materials Research and Testing, Division I.5 Richard-Willstaetter-Str. 11 12489 Berlin Germany
Robert Brehm, BAM Federal Institute for Materials Research and Testing, Division I.5 Richard-Willstaetter-Str. 11 12489 Berlin Germany
Monika Spieles, BAM Federal Institute for Materials Research and Testing, Division I.5 Richard-Willstaetter-Str. 11 12489 Berlin Germany
Werner A. Kaiser, Institut für Diagnostische und Interventionelle Radiologie des Klinikums der Friedrich-Schiller-Universität Jena (IDIR), Forschungszentrum Lobeda Erlanger Allee 101 07747 Jena Germany
Ingrid Hilger, Institut für Diagnostische und Interventionelle Radiologie des Klinikums der Friedrich-Schiller-Universität Jena (IDIR), Forschungszentrum Lobeda Erlanger Allee 101 07747 Jena Germany
Ute Resch-Genger, BAM Federal Institute for Materials Research and Testing, Division I.5 Richard-Willstaetter-Str. 11 12489 Berlin Germany
Fluorescence techniques are widely used as detection methods in a wide range of biological imaging and analytical applications.
The purpose of this work is to determine a measurement method which leads to a comparison between different classes of fluorophores
in term of stability of the fluorescence signal upon thermal treatment cycles. This kind of investigation can determine whether
the fluorophore performance is affected by heating/cooling cycles and to what extent. The fluorophores considered in this
work were organic fluorophores belonging to the family of indocyanine dyes (IRIS3 by Cyanine Technologies S.p.A.) in their
molecular form or encapsulated within silica nanoparticles, and CdSe/ZnS carboxyl quantum dots (Qdots 565 ITK by Invitrogen).
The NIST Standard Reference Material® SRM 1932 fluorescein solution was used in the certified concentration as reference material
in order to evaluate the repeatability of the used spectrofluorimeter. The proposed measurement protocol allows to characterize
all kind of fluorophores upon thermal treatments. This allows direct comparison of their performance under temperature changes,
giving useful guidelines for the selection of the most suitable fluorophore for the envisaged application. Moreover the method
appears to be a promising tool for the characterisation of reference fluorescent materials. The experimental results demonstrate
that each fluorophore class shows a specific behaviour. The experimental data analysis points out an important hysteresis
effect for quantum dots that was not detected for cyanine molecules and was only slightly detected for cyanine doped silica
nanoparticles.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0610-8
Authors
Leonardo Mortati, Istituto Nazionale di Ricerca Metrologica, Department of Thermodynamics, Optical Molecular Spectroscopy Group Strada delle Cacce, 73 10135 Torino Italy
Ivana Miletto, University of Torino Department of Inorganic, Physics and Material Chemistry and NIS Centre of Excellence Via Pietro Giuria, 7 10125 Torino Italy
Gabriele Alberto, University of Torino Department of Inorganic, Physics and Material Chemistry and NIS Centre of Excellence Via Pietro Giuria, 7 10125 Torino Italy
Giuseppe Caputo, University of Torino Department of Inorganic, Physics and Material Chemistry and NIS Centre of Excellence Via Pietro Giuria, 7 10125 Torino Italy
Maria Paola Sassi, Istituto Nazionale di Ricerca Metrologica, Department of Thermodynamics, Optical Molecular Spectroscopy Group Strada delle Cacce, 73 10135 Torino Italy
The conformational transitions in an oligomeric and high molecular weight class II α-mannosidase from Aspergillus fischeri were examined using fluorescence and CD spectroscopy under chemical, thermal and acid denaturing conditions. The enzyme lost
the activity first and then the overall folded conformation and secondary structure. The midpoint values of GdnHCl mediated
changes measured by inactivation; fluorescence and negative ellipticity were 0.48 M, 1.5 M and 1.9 M, respectively. The protein
almost completely unfolded in 4.0 M GdnHCl but not at 90 °C. The inactivation and unfolding were irreversible. At pH 2.0,
the protein exhibited molten-globule like intermediate with rearranged secondary and tertiary structures and exposed hydrophobic
amino acids on the surface. This species showed increased accessibility of Trp to the quenchers and got denatured with GdnHCl
in a different manner. The insoluble aggregates of a thermally denatured protein could be detected only in the presence of
0.25–0.75 M GdnHCl.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0625-1
Authors
K. S. Shashidhara, National Chemical Laboratory Biochemical Sciences Division Dr.Homi Bhabha road Pune 411008 India
Sushama M. Gaikwad, National Chemical Laboratory Biochemical Sciences Division Dr.Homi Bhabha road Pune 411008 India
This paper reports on the spectroscopic properties and energy transfer analysis of Tm3+-doped BaF2-Ga2O3-GeO2-La2O3 glasses with different Tm2O3 doping concentrations (0.2, 0.5, 2.0, 2.5, 3.0, 3.5, 3.5, 4.0 wt%). Mid-IR fluorescence intensities in the range of 1,300 nm−2,200 nm
have been measured when excited under an 808 nm LD for all the samples with the same pump power. Energy level structure and
Judd-Ofelt parameters have been calculated based on the absorption spectra of Tm3+, cross-relaxation rates and multi-phonon relaxation rates have been estimated with different Tm2O3 doping concentrations. The maximum fluorescence intensity at around 1.8 μm has been obtained in Tm2O3-3 wt% sample and the maximum value of calculated stimulated emission cross-section of Tm3+ in this sample is about 0.48 × 10−20 cm2 at 1,793 nm, and there is not any crystallization peak in the DSC curve of this sample, which indicate the potential utility
of Tm3+-doped BaF2-Ga2O3-GeO2- La2O3 glass for 2.0-μm optical fiber laser.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0617-1
Authors
Shenglei Yu, South China University of Technology Institute of Optical Communication Materials, Key Laboratory of Special Functional Materials of Ministry of Education Guangzhou 510641 People’s Republic of China
Zhongmin Yang, South China University of Technology Institute of Optical Communication Materials, Key Laboratory of Special Functional Materials of Ministry of Education Guangzhou 510641 People’s Republic of China
Shanhui Xu, South China University of Technology Institute of Optical Communication Materials, Key Laboratory of Special Functional Materials of Ministry of Education Guangzhou 510641 People’s Republic of China
Imidacloprid belongs to a major new class of insecticides, called neonicotinoids, which are accounting for 11–15% of the total
insecticide market. The binding characteristics of insecticide imidacloprid with hemoglobin (Hb) have been studied by employing
different spectroscopic techniques. The results proved the formation of complex between imidacloprid and Hb. Hydrophobic interaction
and hydrogen bond dominated in the association reaction. Hydrophobic probe 8-anilino-1-naphthalenesulfonic acid (ANS) competitive
experiments indicated that the binding of imidacloprid to Hb primarily took place in hydrophobic regions. The distance between
Hb donor and acceptor imidacloprid was 4.88 nm as derived from Förster’s theory. Alternations of Hb secondary structure in
the presence of imidacloprid were confirmed by synchronous fluorescence, circular dichroism (CD) and three-dimensional fluorescence
spectra. This study enriches our understanding of toxic effect of imidacloprid to the physiologically important protein Hb.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0618-0
Authors
Fei Ding, China Agricultural University Department of Chemistry No. 2 Yuanmingyuan Xi Road Haidian District Beijing China 100193
Bin-Yue Han, China Agricultural University College of Biological Sciences Beijing 100193 China
Wei Liu, China Agricultural University Department of Chemistry No. 2 Yuanmingyuan Xi Road Haidian District Beijing China 100193
Li Zhang, China Agricultural University Department of Chemistry No. 2 Yuanmingyuan Xi Road Haidian District Beijing China 100193
Ying Sun, China Agricultural University Department of Chemistry No. 2 Yuanmingyuan Xi Road Haidian District Beijing China 100193
The spectroscopic properties of 2-[4′-(dimethylamino)phenyl]-benzothiazole (BTA-2) in solution and in the presence of amyloid
fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles
in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH3CN). BTA-2 binds to amyloid fibrils in solution leading to a characteristic blue-shift in the emission spectrum and an increase
in fluorescence intensity. However, in solutions with increasing CH3CN concentration, there was a marked decrease in binding of the BTA-2 to fibrils. Studies demonstrating the effect of BTA-2
concentration on binding were performed. A comparison with the standard amyloid fluorescent marker, thioflavin T (ThT), showed
that BTA-2 is more fluorescent, making it an excellent dye to label amyloid samples.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0634-0
Authors
Catherine C. Kitts, University of Texas at Austin Department of Chemistry and Biochemistry, Center for Nano and Molecular Science and Technology, and Texas Materials Institute Austin TX 78712 USA
David Anton Vanden Bout, University of Texas at Austin Department of Chemistry and Biochemistry, Center for Nano and Molecular Science and Technology, and Texas Materials Institute Austin TX 78712 USA
It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU) - cetyltrimethylammonium
bromide (CTAB) – nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence
intensity is proportion to the concentration of nucleic acids in the range of 2.0 × 10−8–1.0 × 10−6 g mL−1 for fish sperm DNA (fsDNA), 2.0 × 10−8–1.0 × 10−6 g mL−1 for calf thymus DNA (ctDNA), 1.0 × 10−8–1.0 × 10−6 g mL−1 for yeast RNA (yRNA), and their detection limits (S/N = 3) are 8.0 ng mL−1, 10.5 ng mL−1 and 5.8 ng mL−1, respectively. This method is used for determining the concentration of DNA in actual sample with satisfactory results. The
interaction mechanism is also studied.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0627-z
Authors
Haiping Zhou, Shandong University Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering Jinan 250100 China
Xia Wu, Shandong University Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering Jinan 250100 China
Wei Xu, Shandong University Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering Jinan 250100 China
Jinghe Yang, Shandong University Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering Jinan 250100 China
Qiuxia Yang, University of Jinan School of Chemistry and Chemical Engineering Jinan 250022 China
The complex of Tb(TPTZ)Cl3·3H2O was synthesized by adding the ethyl alcohol solution of TbCl3 (1 mmol) to the solution of 2,4,6-tris-(2-pyridyl)-s-triazine(TPTZ,1 mmol) with constant stirring. The solution which had
been filtered was kept at the room temperature for 4 weeks, and then a kind of transparent crystal was formed. Besides, nine
kinds of solid complexes in the different molar proportion of terbium to gadolinium had been synthesized by adopting the similar
method mentioned above. It was inferred from the elemental analysis and rare earth complexometry that the composition of these
complexes is (TbxGdy)(TPTZ)Cl3·3H2O (x : y = 0.9 : 0.1, 0.8 : 0.2, 0.7 : 0.3, 0.6 : 0.4, 0.5 : 0.5, 0.4 : 0.6, 0.3 : 0.7, 0.2 : 0.8, 0.1 : 0.9). The absorption
spectra and photoluminescence of the complexes were determined in dimethylsulfoxide (DMF), which showed that the excitation
of the complexes is mostly ligand based. The triplet state energy level of TPTZ was measured, indicating that the lowest excitation
state energy level of Tb(III) and the triplet state energy level of TPTZ match well each other. The fluorescent data indicated
that the fluorescent emission intensity of Tb3+ ions would be enhanced in the complexes after terbium was doped with Gd3+ ion. When x : y was 0.5 : 0.5, the fluorescent emission intensity was the largest. The result obtained by testing the X-ray
diffraction of the monocrystal revealed that the molecular formula of the mono-crystal complex is [Tb(TPTZ)(H2O)6]Cl3·3H2O. The number of metal ion coordinates is nine, and the tridentate TPTZ and six water molecules are bonded with terbium respectively.
Besides, it also revealed that the monocrystal belongs to the monoclinic system, and space group Cc with the following unit
cell parameters is a = 1.4785 (3) nm, b = 1.0547 (2) nm, c = 1.7385 (4) nm, β = 94.42 (3)°, V = 2.7028 (9) nm3 and Z = 4.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0619-z
Authors
Yan-Fang Zhao, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Yong-Liang Zhao, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Feng Bai, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Xiao-yan Wei, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Yong-sheng Zhou, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Mei-na Shan, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Huan-huan Li, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Rui-jun Ma, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Xiao-tao Fu, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Yan Du, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 China
Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were
studied. The dyes enhance their fluorescence intensity in 2–3 orders of magnitude upon interaction with DNA. Studied styrylcyanines
in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the
values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy
of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably
brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids-
containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl
DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0630-4
Authors
Valentyna P. Tokar, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Mykhaylo Yu. Losytskyy, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Tymish Y. Ohulchanskyy, SUNY at Buffalo Institute for Lasers, Photonics and Biophotonics Buffalo NY 14260 USA
Dmytro V. Kryvorotenko, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Vladyslava B. Kovalska, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Anatoliy O. Balanda, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Igor M. Dmytruk, Physics Faculty, Kyiv Taras Shevchenko National University Build.1, academic Glushkov Ave., 2 03680 Kyiv Ukraine
Vadym M. Prokopets, Physics Faculty, Kyiv Taras Shevchenko National University Build.1, academic Glushkov Ave., 2 03680 Kyiv Ukraine
Sergiy M. Yarmoluk, Institute of Molecular Biology and Genetics of NASU 150 Zabolotnogo Str. 03143 Kyiv Ukraine
Valeriy M. Yashchuk, Physics Faculty, Kyiv Taras Shevchenko National University Build.1, academic Glushkov Ave., 2 03680 Kyiv Ukraine
Fluorescence properties of four new potential antitumoral compounds, 3-arylbenzothieno[2,3-c]pyran-1-ones, were studied in solution and in lipid membranes of dipalmitoyl phosphatidylcholine (DPPC), egg yolk phosphatidylcholine
(Egg-PC) and dioctadecyldimethylammonium bromide (DODAB). The 3-(4-methoxyphenyl)benzothieno[2,3-c]pyran-1-one (1c) exhibits the higher fluorescence quantum yields in all solvents studied. All compounds present a solvent sensitive emission,
with significant red shifts in polar solvents for the methoxylated compounds. The results point to an ICT character of the
excited state, more pronounced for compound 1c. Fluorescence (steady-state) anisotropy measurements of the compounds incorporated in liposomes of DPPC, DODAB and Egg-PC
indicate that all compounds have two different locations, one due to a deep penetration in the lipid membrane and another
corresponding to a more hydrated environment. In general, the methoxylated compounds prefer hydrated environments inside the
liposomes. The 3-(4-fluorophenyl)benzothieno[2,3-c]pyran-1-one (1a) clearly prefers a hydrated environment, with some molecules located at the outer part of the liposome interface. On the
contrary, the preferential location of 3-(2-fluorophenyl)benzothieno[2,3-c]pyran-1-one (1b) is in the region of lipid hydrophobic tails. Compounds with a planar geometry (1a and 1c) have higher mobility in the lipid membranes when phase transition occurs.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0607-3
Authors
Elisabete M. S. Castanheira, Universidade do Minho Centro de Física (CFUM) Campus de Gualtar 4710-057 Braga Portugal
M. Solange D. Carvalho, Universidade do Minho Centro de Física (CFUM) Campus de Gualtar 4710-057 Braga Portugal
Daniel J. G. Soares, Universidade do Minho Centro de Física (CFUM) Campus de Gualtar 4710-057 Braga Portugal
Paulo J. G. Coutinho, Universidade do Minho Centro de Física (CFUM) Campus de Gualtar 4710-057 Braga Portugal
Ricardo C. Calhelha, Universidade do Minho Centro de Química (CQ-UM) Campus de Gualtar 4710-057 Braga Portugal
Maria-João R. P. Queiroz, Universidade do Minho Centro de Química (CQ-UM) Campus de Gualtar 4710-057 Braga Portugal
In pH 1.8 ∼ 2.8 weak acid medium, polyvinylpyrrolidone (PVP) and Eosin Y reacted to form complex that could result in Eosin
Y (EY) fluorescence quenching. The maximum quenching wavelength was at 542 nm. The fluorescence quenching (ΔF) was proportional to the concentration of polyvinylpyrrolidone in a certain range. The linear range, the correlation coefficient
and the detection limit were 0.33 ∼ 2.0 μg•mL−1, 0.9994 and 99.6 ng•mL−1, respectively. The influences of the coexistence substances were tested and the results showed that the method had good selectivity.
Therefore, a new method based on fluorescence quenching of eosin Y by PVP for the determination of trace PVP was developed.
The method was sensitive, simple and rapid, which was applied to the determination of trace PVP in the beer with satisfactory
results. The reaction mechanism was also discussed.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0615-3
Authors
Lihong Yu, Southwest University School of Chemistry and Chemical Engineering Chongqing 400715 China
Zhongfang Liu, Southwest University School of Chemistry and Chemical Engineering Chongqing 400715 China
Xiaoli Hu, Southwest University School of Chemistry and Chemical Engineering Chongqing 400715 China
Ling Kong, Southwest University School of Chemistry and Chemical Engineering Chongqing 400715 China
Shaopu Liu, Southwest University School of Chemistry and Chemical Engineering Chongqing 400715 China
The rotational diffusion of three probes: coumarin 522B (C522B), coumarin 307 (C307) and coumarin 138 (C138) with nearly identical
size was studied at room temperature employing steady-state and time-resolved fluorescence anisotropy techniques in series
of alcohols and alkanes. Experimental observations indicate faster rotation of C138 compared to the other two dyes in alcohols
and a faster rotation of C522B than C307 in alkanes. The dielectric friction theories of Nee-Zwanzig (NZ) and van der Zwan-Hynes
(ZH) were employed to estimate the friction experienced by the probes in alcohols, in addition to the mechanical friction
calculated using Stokes-Einstein-Debye (SED) hydrodynamic with slip boundary condition and Dote-Kivelson-Schwartz (DKS) quasihydrodynamic
theories. The observed reorientation times for the three probes do not follow the trend predicted by dielectric friction theories
of NZ and ZH. The dipole moments determined from solvatochromic techniques were found to be different for the three probes.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0606-4
Authors
J. R. Mannekutla, Karnatak University Laser Spectroscopy Programme, Department of Physics Dharwad 580 003 India
Sanjeev R. Inamdar, Karnatak University Laser Spectroscopy Programme, Department of Physics Dharwad 580 003 India
B. G. Mulimani, Gulbarga University Gulbarga 585 106 India
M. I. Savadatti, Karnataka State Council for Higher Education Bangalore 560 001 India
Human serum albumin consists of a single polypeptide of 585 amino acid residues with 1 Trp residue. In the present work, we
measured fluorescence lifetimes of the protein in both native and denatured states. The results indicate that Trp emission
occurs with three lifetimes in both states. Lifetimes values and contribution to the global emission decay differ between
the two states. Data are interpreted as the results of an emission occurring from three substructures of the tryptophan formed
in the excited state. Two of these substructures are already present for the tryptophan free in solution. The third lifetime
is the result of the interaction between the tryptophan residue and surrounding microenvironment. The populations of these
substructures characterized by the pre-exponential parameters of the fluorescence lifetimes are dependent on the fluorophore
microenvironment and on the global protein structure.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0597-1
Authors
Megdouda Amiri, Université des Sciences et Technologies de Lille Laboratoire de Biophysique Moléculaire Bâtiment C6. 59655 Villeneuve d’Ascq Cédex France
Kristina Jankeje, Université des Sciences et Technologies de Lille Laboratoire de Biophysique Moléculaire Bâtiment C6. 59655 Villeneuve d’Ascq Cédex France
Jihad René Albani, Université des Sciences et Technologies de Lille Laboratoire de Biophysique Moléculaire Bâtiment C6. 59655 Villeneuve d’Ascq Cédex France
The metal-ligand complex, [Ru(2,2′-bipyridine)2(4,4′-dicarboxy-2,2′-bipyridine)]2+ (RuBDc), was used as a spectroscopic probe for studying macromolecular dynamics. RuBDc is a very photostable probe that possesses
favorable photophysical properties including long lifetime, high quantum yield, large Stokes’ shift, and highly polarized
emission. To further show the usefulness of this luminophore for probing macromolecular dynamics, we examined the intensity
and anisotropy decays of RuBDc when conjugated to R17 bacteriophage using frequency-domain fluorometry with a blue light-emitting
diode (LED) as the modulated light source. The intensity decays were best fit by a sum of two exponentials, and we obtained
a longer mean lifetime at 4 °C (<τ> = 491.8 ns) as compared to that at 25 °C (<τ> = 435.1 ns). The anisotropy decay data showed
a single rotational correlation time, which is typical for a spherical molecule, and the results showed a longer rotational
correlation time at 4 °C (2,574.9 ns) than at 25 °C (2,070.1 ns). The use of RuBDc enabled us to measure the rotational correlation
time up to several microseconds. These results indicate that RuBDc has significant potential for studying hydrodynamics of
biological macromolecules.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0612-6
Authors
Myung Sup Kim, Pusan National University Department of Oral Biochemistry and Molecular Biology, School of Dentistry Yangsan 626-870 Korea
Jae Hui Kim, Pusan National University Department of Oral Biochemistry and Molecular Biology, School of Dentistry Yangsan 626-870 Korea
Beng Whwa Son, Pukyong National University Department of Chemistry Busan 608-737 Korea
Jung Sook Kang, Pusan National University Department of Oral Biochemistry and Molecular Biology, School of Dentistry Yangsan 626-870 Korea
A simple, sensitive, accurate and affordable spectrofluorimetric method was developed and validated for the determination
of venlafaxine, both in marketed preparations as well as in spiked rat plasma. Venlafaxine depicted strong native fluorescence
property in freshly prepared 0.05 M sulphuric acid. The excitation and emission wavelengths were found to be 237.0 nm and
301.0 respectively. Effect of variations in pH, temperature, concentration, change in molarities of different solvents, and
effect of excipients were studied. The calibration graph in case of dosage forms and in spiked plasma was found to be rectilinear
in the concentrations of 15–600 ng/ml and 20–650 ng/ml respectively. The intra- day and inter-day accuracy measurements of
VEN in formulations ranged from 0.29 to 0.44% and 0.27 to 0.49%, respectively. The intra-day and inter-day accuracy in measurement
of VEN in plasma ranged from 0.062 to 2.26% and 0.52 to 2.32%, respectively. The limit of detection (LOD) was found to be
6.0 ng/mL and 4.0 ng/mL in plasma and formulations respectively. The mean recovery of VEN from plasma was 97.46.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0624-2
Authors
Sheikh Shahnawaz, Lloyd College Department of Pharmacy Knowledge park II Greater Noida 201306 India
Zaki Siddiqui, Dreamz College of Pharmacy Mandi Himachal Pradesh India
Quaisul Hoda, Lloyd College Department of Pharmacy Knowledge park II Greater Noida 201306 India
Self-assembly of tetracationic porphyrin TMPyP4+ onto polyanionic matrix of inorganic polyphosphate (PPS) in aqueous solutions has been studied in a wide range of molar phosphate-to-dye
ratios using techniques of polarized fluorescence, absorption, resonance Raman spectroscopy and static light scattering. The
binding of TMPyP4+ to PPS is characterized by the binding constant of 3 × 105 M−1 and the cooperativity parameter of about 150. The fluorescence quenching of the bound TMPyP4+ evidences the stacking of the porphyrine chromophores. Under the stoichiometric binding ratio TMPyP4+ forms extended continuous face-to-face aggregates (so-called H-aggregates) which manifest themselves by a blue shift (12 nm)
and a large hypochromisity (51%) of the Soret absorption band. Each face-to-face TMPyP4+ stack is formed with participation of four PPS chains. Formation of such columnar aggregates is promoted by the ability of
PPS chains to take a helix conformation where negative charges are arranged along two oppositely situated rows with intercharge
distance of 0.36 nm which corresponds to the thickness of the porphyrin π-electronic system. The ability of each PPS strand
to be template for formation of two porphyrin stacks results in the integration of the adjacent stacks into higher-order aggregates
which dimension was estimated from the fluorescence polarization data.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0609-1
Authors
Victor N. Zozulya, B. Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine Department of Molecular Biophysics 47 Lenin ave. 61103 Kharkov Ukraine
Olga A. Ryazanova, B. Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine Department of Molecular Biophysics 47 Lenin ave. 61103 Kharkov Ukraine
Igor M. Voloshin, B. Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine Department of Molecular Biophysics 47 Lenin ave. 61103 Kharkov Ukraine
Alexandr Yu. Glamazda, B. Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine Department of Molecular Biophysics 47 Lenin ave. 61103 Kharkov Ukraine
Victor A. Karachevtsev, B. Verkin Institute for Low Temperature Physics & Engineering of NAS of Ukraine Department of Molecular Biophysics 47 Lenin ave. 61103 Kharkov Ukraine
Prerequisites for successful flow cytometry investigations are specific antibodies labeled with appropriate fluorochromes
and negligible autofluorescence of the untreated cells at the wavelength of interest. The aim of this study was (a) to characterize
frequently used urological carcinoma cell lines with regard to their autofluorescence properties, (b) to demonstrate the autofluorescence
as a serious interfering factor on FACS analysis of urological carcinoma cell lines and (c) to suggest an alternative to avoid
interfering autofluorescence. Twenty-one cell lines originating from prostate carcinoma, renal cell carcinoma and bladder
cancer were included in this study. The various cell lines were read on a flow cytometer in comparison to human erythrocytes
as cells with low fluorescence intensity. Urological cell lines show a high autofluorescence when flow cytometry analyses
are performed at the frequently used excitation wavelengths at 405 and 488 nm. At excitation wavelength of 633 nm, this problem
was reduced and most of the cell lines (14/21) were without autofluorescence at the emission wavelength of 785 nm. In addition,
with a spectrofluorometer three exemplary cell lysates were investigated. The above observations were confirmed. The dye APC-Cy7
is one suitable fluorochrome for successful investigation under these measurement conditions.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0621-5
Authors
Angelika Tölle, Charité–Universitätsmedizin Berlin Department of Urology Charitéplatz 1 10117 Berlin Germany
Ziyad Abdallah, Charité—Universitätsmedizin Berlin Institute of Transfusion Medicine 10117 Berlin Germany
Klaus Jung, Berlin Institute for Urologic Research 10117 Berlin Germany
Hans Bäumler, Charité—Universitätsmedizin Berlin Institute of Transfusion Medicine 10117 Berlin Germany
A rapid and efficient method for the synthesis of novel dipyrazolo[3,4-b:3′,4′-d]pyridines (DPP) from pyrazolo[3,4-b]pyridine was successfully developed. The DPP derivative was further N-alkylated (6, 8) as well as N-linked with amino acids (13) and their photophysical properties were studied along with N-aryl DPP 4 and observed that the chromophores at C4 position in the aryl ring changed the absorption and emission λmax.
Content Type Journal Article
Category Rapid Communication
DOI 10.1007/s10895-010-0622-4
Authors
Madhukar N. Jachak, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Sandeep M. Bagul, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Dilip R. Birari, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Maruti G. Ghagare, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Muddassar A. Kazi, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Raghunath B. Toche, B.H. Commerce and A.M. Science College Organic Chemistry Research Center, Department of Chemistry, K.R.T. Arts Gangapur Road Nashik 422002 MS India
Vijayavitthal T. Mathad, Mega Fine Pharma Pvt. Ltd. 201, Lakhmapur, Dindori Nashik 422202 MS India
A coumarin-based fluorescent chemosensor 1 for Zn2+ was designed and synthesized. Compound 1 exhibits lower background fluorescence due to intramolecular photoinduced electron transfer. However, upon mixing with Zn2+ in 30% (v/v) aqueous ethanol, a “turn-on” fluorescence emission is observed. The fluorescence emission increases linearly with Zn2+ concentration in the range 0.5–10 μmol L−1 with a detection limit of 0.29 μmol L−1. No remarkable emission enhancement was, however, observed for other metal ions. The proposed chemosensor was applied to
the determination of Zn2+ in water samples with satisfactory results.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0628-y
Authors
Zhuan Su, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Kangyu Chen, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Yuan Guo, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Haiping Qi, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Xiao-Feng Yang, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Minglei Zhao, Northwest University Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Department of Chemistry Xi’an 710069 China
Luminescent zinc-based hybrid inorganic–organic films with rare--earth (RE) complexes have been prepared using a non-alkoxide
sol–gel process. The films were fabricated by the dip-coating method starting from zinc acetate dihydrate, rare earth chloride,
lactic acid as hydrolytic catalyst, and anhydrous ethanol. The β-diketones thenoylltrifluoroacetone (Httfa) and dibenzoylmethane
(Hdbm) were used as ligands to Eu3+ and Tb3+, respectively. After deposition of the first layer, the films were fired at temperatures between 50 and 300 °C, in air. Photophysical
properties such as excitation, emission and emission, lifetimes were determined for the films obtained in different conditions.
Eu3+/ttfa and Tb3+/dbm films fired at 300 and 250 °C, respectively, are still transparent and gave rise to intense emission when excited through
the ligand (antenna effect).
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0616-2
Authors
Renata Figueredo Martins, FFCLRP-USP Department of Chemistry Av. dos Bandeirantes, 3900 CEP 14040-901 Ribeirão Preto SP Brazil
Rodrigo Ferreira Silva, FFCLRP-USP Department of Chemistry Av. dos Bandeirantes, 3900 CEP 14040-901 Ribeirão Preto SP Brazil
Rogéria Rocha Gonçalves, FFCLRP-USP Department of Chemistry Av. dos Bandeirantes, 3900 CEP 14040-901 Ribeirão Preto SP Brazil
Osvaldo Antonio Serra, FFCLRP-USP Department of Chemistry Av. dos Bandeirantes, 3900 CEP 14040-901 Ribeirão Preto SP Brazil
The use of hydrophobic fluorescent probe ABM (benzanthrone derivative) and albumin autofluorescence allowed show conformational
alterations in Chernobyl clean-up workers blood plasma. Results obtained in 1996–1997 suggest that acidic expansion of plasma
albumin takes place. Latest data (2006–2008) result in splitting of albumin alterations onto two stages - acidic expansion
and N-F transition. The N-F transition is accompanied by the blue shift of fluorescence spectra and dehydration of tryptophanyl
region of albumin molecule. In 2007 obtained.patterns of ABM spectra had never been previously seen in examined healthy individuals
or patients with tuberculosis, multiple sclerosis, rheumatoid arthritis, etc. Patterns of ABM fluorescence spectra are associated
with conformational changes of blood plasma albumin. The use of probe ABM and albumin auto-fluorescence allowed show conformational
alterations in albumin of Chernobyl clean-up workers blood plasma. It is necessary to note that all investigated parameters
significantly differ in observed groups of patients. These findings reinforce our understanding that the blood plasma albumin
is a significant biological target of radiation. It may be concluded that fluorescence characteristics are representative
of radiation induced albumin alterations and its carrier function.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0608-2
Authors
Elena M. Kirilova, Daugavpils University Daugavpils Latvia
Inta Kalnina, Daugavpils University Daugavpils Latvia
Tija Zvagule, Riga Stradins University Riga Latvia
Natalija Gabruseva, University of Latvia Biomedical Research and Study Centre Riga Latvia
Natalja Kurjane, Riga Stradins University Riga Latvia
Irina I. Solomenikova, Daugavpils University Daugavpils Latvia
Two novel ternary rare earth complexes of Tb(III) and Dy(III) perchlorates with bis(benzoylmethyl) sulfoxide (L) and benzoic
acid (L′) had been synthesized and characterized by elemental analysis, coordination titration analysis, molar conductivity,
IR, TG-DSC, 1HNMR and UV spectra. The results indicated that the composition of these complexes was REL5L′(ClO4)2·nH2O (RE= Tb(III), Dy(III); L=C6H5COCH2SOCH2COC6H5, L′=C6H5COO; n = 6,8). The fluorescence spectra illustrated that the ternary rare earth complexes presented stronger fluorescence intensities,
longer lifetimes and higher fluorescence quantum efficiencies than the binary rare earth complexes REL5·(ClO4)3·2H2O. After the introduction of the second ligand benzoic acid group, the relative fluorescence emission intensities and fluorescence
lifetimes of the ternary complexes REL5L′(ClO4)2·nH2O (RE= Tb(III), Dy(III)) enhanced more obviously than the binary complexes. This indicated that the presence of both organic
ligands bis(benzoylmethyl) sulfoxide and the second ligand benzoic acid could sensitize fluorescence intensities of rare earth
ions, and the introduction of benzoic acid group was resulted in the enhancement of the fluorescence properties of the ternary
rare earth complexes. The phosphorescence spectra were also discussed.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0633-1
Authors
Wen-Xian Li, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 People’s Republic of China
Wen-Juan Chai, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 People’s Republic of China
Xiao-Jun Sun, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 People’s Republic of China
Tie Ren, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 People’s Republic of China
Xiao-Yan Shi, Inner Mongolia University College of Chemistry and Chemical Engineering Hohhot 010021 People’s Republic of China
The in-situ, non-contact, and non-destructive measurement of the physicochemical properties such as the polarity of thin, hydrophilic
polymer films is desirable in many areas of polymer science. Polarity is a complex factor and encompasses a range of non-covalent
interactions including dipolarity/polarizability and hydrogen bonding. A polarity measurement method based on fluorescence
would be ideal, but the key challenge is to identify suitable probes which can accurately measure specific polarity related
parameters. In this manuscript we assess a variety of fluorophores for measuring the polarity of a series of relatively hydrophilic,
thermoresponsive N-isopropylacrylamide/N-tert-butylacrylamide (NIPAM/NtBA) copolymers. The emission properties of both pyrene
and 3-Hydroxyflavone (3-HF) based fluorophores were measured in dry polymer films. In the case of pyrene, a relatively weak,
linear relationship between polymer composition and the ratio of the first to the third vibronic band of the emission spectrum
(I1/I3) is observed, but pyrene emission is very sensitive to temperature and thus not suitable for robust polarity measurements.
The 3-HF fluorophores which can undergo an excited-state intramolecular proton transfer (ESIPT) reaction have a dual band
fluorescence emission that exhibits strong solvatochromism. Here we used 4′-diethylamino-3-hydroxyflavone (FE), 5,6-benzo-4′-diethylamino-3-hydroxyflavone
(BFE), and 4´-diethylamino-3-hydroxy-7-methoxyflavone (MFE). The log ratio of the dual band fluorescence emission (log (IN*/IT*)) of 3-HF doped, dry, NIPAM-NtBA copolymer films were found to depend linearly on copolymer composition, with increasing
hydrophobicity (greater NtBA fraction) leading to a decrease in the value of log (IN*/IT*). However, the ESIPT process in the polymer matrix was found to be irreversible, non-equilibrated and occurs over a much
longer timescale in comparison to the results previously reported for liquid solvents.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0613-5
Authors
Boguslaw Szczupak, National University of Ireland, Galway Nanoscale Biophotonics Laboratory, School of Chemistry Galway Ireland
Alan G. Ryder, National University of Ireland, Galway Nanoscale Biophotonics Laboratory, School of Chemistry Galway Ireland
Denisio M. Togashi, National University of Ireland, Galway Nanoscale Biophotonics Laboratory, School of Chemistry Galway Ireland
Andrey S. Klymchenko, Université de Strasbourg Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Faculté de Pharmacie Illkirch France
Yuri A. Rochev, National University of Ireland, Galway National Centre for Biomedical Engineering Sciences Galway Ireland
Alexander Gorelov, University College Dublin Department of Chemistry Dublin Ireland
Thomas J. Glynn, National University of Ireland, Galway School of Physics Galway Ireland
The progression to end-stage renal failure is independent of the initial pathogenic mechanism. Metabolic acidosis is a common
consequence of chronic renal failure that results from inadequate ammonium excretion and decreased tubular bicarbonate reabsorption.
Protoporphyrin IX (PpIX) is the immediate metabolic precursor of the heme molecule. The purpose of this study was to evaluate
the levels of erythrocytes protoporphyrin IX at an animal model during progressive renal disease. A total of 36 eight-week-old
male Wistar rats were divided into six groups: Normal, 4 and 8 weeks after 5/6 nephrectomy (NX). Renal function was evaluated
by creatinine clearance and plasma creatinine levels. The autofluorescence of erythrocytes porphyrin of healthy and NX rats
was analyzed using fluorescence spectroscopy. Emission spectra were obtained by exciting the samples at 405 nm. Significant
differences between normal and NX rats autofluorescence shape occurred in the 600–700 nm spectral region. A correlation was
observed between emission band intensity at 635 nm and progression of renal disease.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0600-x
Authors
Vivian Regina Tristão, Universidade Federal de São Paulo—UNIFESP-EPM Disciplina Nefrologia, Departamento de Medicina São Paulo Brazil
Fernando Felippe de Carvalho, Universidade Federal de São Paulo—UNIFESP-EPM Disciplina Nefrologia, Departamento de Medicina São Paulo Brazil
Cinthia Zanini Gomes, IPEN-CNEN-SP Instituto de Pesquisas Energéticas e Nucleares São Paulo Brazil
Adriana Regina Miranda, IPEN-CNEN-SP Instituto de Pesquisas Energéticas e Nucleares São Paulo Brazil
Cíntia C. Vequi-Suplicy, Instituto de Física da Universidade de São Paulo—USP São Paulo Brazil
Maria Teresa Lamy, Instituto de Física da Universidade de São Paulo—USP São Paulo Brazil
Nestor Schor, Universidade Federal de São Paulo—UNIFESP-EPM Disciplina Nefrologia, Departamento de Medicina São Paulo Brazil
Maria Helena Bellini, Universidade Federal de São Paulo—UNIFESP-EPM Disciplina Nefrologia, Departamento de Medicina São Paulo Brazil
The synthesis and characterization of photoactive hybrid materials based on [Ru(bpy)3]2+ physically adsorbed within the channels of mesoporous MCM-41 silica nanoparticles is presented. A set of photoactive mesostructured
hybrids with different guest loading has been prepared and characterized by X-ray diffraction, High Resolution Transmission
Electron Microscopy, volumetric analyses, Diffuse Reflectance UV-Vis and Photoluminescence spectroscopies and lifetime measurements.
The hybrids synthesis and the washing procedures, performed to investigate the host-guest interaction and the stability of
the complex within the mesopores, didn’t affect the integrity of the structure and morphology of MCM-41 nanoparticles. The
dispersion of [Ru(bpy)3]2+ within the channels varied depending on the loading value and this is reflected in the different and peculiar photoluminescence
features of the resulting hybrid materials. Photoluminescence spectroscopy evidenced that the use of MCM-41 nanoparticles
ensures a better dispersion of the complex within the mesopores, if compared with traditional MCM-41. Further studies are
in progress to investigate the interesting and promising features exhibited by such photoactive systems for advanced applications
of electrochemiluminescence in optoelectronics and diagnostics.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0605-5
Authors
Emanuela Bottinelli, University of Turin Dipartimento di Chimica IFM and NIS, Centre of Excellence via P. Giuria 7 10125 Torino Italy
Ivana Miletto, University of Turin Dipartimento di Chimica IFM and NIS, Centre of Excellence via P. Giuria 7 10125 Torino Italy
Giuseppe Caputo, University of Turin Dipartimento di Chimica IFM and NIS, Centre of Excellence via P. Giuria 7 10125 Torino Italy
Salvatore Coluccia, University of Turin Dipartimento di Chimica IFM and NIS, Centre of Excellence via P. Giuria 7 10125 Torino Italy
Enrica Gianotti, University of Turin Dipartimento di Chimica IFM and NIS, Centre of Excellence via P. Giuria 7 10125 Torino Italy
SrMoO4 doped with rare earth are still scarce nowadays and have attracted great attention due to their applications as scintillating
materials in electro-optical like solid-state lasers and optical fibers, for instance. In this work Sr1−xEuxMoO4 powders, where x = 0.01; 0.03 and 0.05, were synthesized by Complex Polymerization (CP) Method. The structural and optical
properties of the SrMoO4:Eu3+ were analyzed by powder X-ray diffraction patterns, Fourier Transform Infra-Red (FTIR), Raman Spectroscopy, and through Photoluminescent
Measurements (PL). Only a crystalline scheelite-type phase was obtained when the powders were heat-treated at 800 °C for 2 h,
2θ = 27.8° (100% peak). The excitation spectra of the SrMoO4:Eu3+ (λEm. = 614 nm) presented the characteristic band of the Eu3 + 5L6 transition at 394 nm and a broad band at around 288 nm ascribed to the charge-transfer from the O (2p) state to the Mo (4d)
one in the SrMoO4 matrix. The emission spectra of the SrMoO4:Eu3+ powders (λExc. = 394 and 288 nm) show the group of sharp emission bands among 523–554 nm and 578–699 nm, assigned to the 5D1→7F0,1and 2 and 5D0→7F0,1,2,3 and 4, respectively. The band related to the 5D0→7F0 transition indicates the presence of Eu3+ site without inversion center. This hypothesis is strengthened by the fact that the band referent to the 5D0→7F2 transition is the most intense in the emission spectra.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0604-6
Authors
Ana Paula A. Marques, Universidade Federal de São Carlos Laboratório Interdisciplinar de Eletroquímica e Cerâmica, Centro Multidisciplinar de Desenvolvimento de Materiais Cerâmicos, Departamento de Química C. Postal 676 13565-905 São Carlos SP Brazil
Marcos Takashi S. Tanaka, Universidade Federal de São Carlos Laboratório Interdisciplinar de Eletroquímica e Cerâmica, Centro Multidisciplinar de Desenvolvimento de Materiais Cerâmicos, Departamento de Química C. Postal 676 13565-905 São Carlos SP Brazil
Elson Longo, Universidade Estadual Paulista CMDMC, LIEC, Instituto de Química 14801-907 Araraquara SP Brazil
Edson R. Leite, Universidade Federal de São Carlos Laboratório Interdisciplinar de Eletroquímica e Cerâmica, Centro Multidisciplinar de Desenvolvimento de Materiais Cerâmicos, Departamento de Química C. Postal 676 13565-905 São Carlos SP Brazil
Ieda Lucia Viana Rosa, Universidade Federal de São Carlos Laboratório Interdisciplinar de Eletroquímica e Cerâmica, Centro Multidisciplinar de Desenvolvimento de Materiais Cerâmicos, Departamento de Química C. Postal 676 13565-905 São Carlos SP Brazil
Low-Density Lipoprotein (LDL), often known as “bad cholesterol” is one of the responsible to increase the risk of coronary
arterial diseases. For this reason, the cholesterol present in the LDL particle has become one of the main parameters to be
quantified in routine clinical diagnosis. A number of tools are available to assess LDL particles and estimate the cholesterol
concentration in the blood. The most common methods to quantify the LDL in the plasma are the density gradient ultracentrifugation
and nuclear magnetic resonance (NMR). However, these techniques require special equipments and can take a long time to provide
the results. In this paper, we report on the increase of the Europium emission in Europium-oxytetracycline complex aqueous
solutions in the presence of LDL. This increase is proportional to the LDL concentration in the solution. This phenomenum
can be used to develop a method to quantify the number of LDL particles in a sample. A comparison between the performances
of the oxytetracycline and the tetracycline in the complexes is also made.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0602-8
Authors
Luciane dos Santos Teixeira, IPEN/CNEN-SP Centro de Lasers e Aplicações São Paulo SP Brazil
Andréa Nastri Grasso, IPEN/CNEN-SP Centro de Lasers e Aplicações São Paulo SP Brazil
Andrea Moreira Monteiro, Universidade de São Paulo Instituto de Ciências Biomédicas, ICB–São Paulo São Paulo SP Brazil
Antonio M. Figueiredo Neto, Universidade de São Paulo Instituto de Física São Paulo SP Brazil
Nilson Dias Vieira, IPEN/CNEN-SP Centro de Lasers e Aplicações São Paulo SP Brazil
Magnus Gidlund, Universidade de São Paulo Instituto de Ciências Biomédicas, ICB–São Paulo São Paulo SP Brazil
Juliana Steffens, Universidade de São Paulo Instituto de Ciências Biomédicas, ICB–São Paulo São Paulo SP Brazil
Lilia Coronato Courrol, Universidade Federal de São Paulo—UNIFESP Campus Diadema, Rua Arthur Ridel, 275, Eldorado Diadema SP Brazil
In this article, we presented novel nitro-stilbene derivatives with one or two benzophenone groups as photoinitiators via
multi-steps synthesis. The ultraviolet/visible spectroscopy and the emission spectroscopy of the compounds were determined
in various solvents. The results showed that the ultraviolet/visible absorption spectroscopy of the derivatives with benzophenone
moiety displayed overlap effects of nitro-stilbene and benzophenone parts. In non-polar solvents, the derivatives exhibited
strong emission, while they displayed weak emission in modest and strong polar solvents. Dyes-linked benzopheonone groups
displayed stronger fluorescence emission than simple chromophore parent molecules. Visible-light photoinitiating effects of
the derivatives were investigated extensively. Methyl methacrylate could be photoinitiated efficiently by the derivatives
with benzophenone moieties at very low concentration, even at 1 × 10−5 mol/L. While the photopolymerization efficiency of styrene initiated by the derivatives was lower than that of methyl methacrylate.
Our results showed that the dye-linked photoinitators had more efficient photoinitiating than the simple mixture of dye and
photoinitator. Furthermore, the derivative with two benzophenone groups displayed more excellent phototiniatiating effects
than the derivative with one benzophenone group. Thermodynamics driving for the occurrence of visible-light photoinduced intramolecular
electron transfer from chromophore part to benzophenone part was evaluated. Benzopinacol moiety produced in photoreaction
was confirmed by nuclear magnetic resonant spectroscopy. Thermal stability of the derivatives was analyzed.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0611-7
Authors
Fang Gao, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Jian Liu, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Huayong Peng, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Nvdan Hu, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Hongru Li, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Shengtao Zhang, Chongqing University College of Chemistry and Chemical Engineering Chongqing 400044 China
Abstract Two soybean cultivars, one conventional and a glyphosate-tolerant (transgenic), were submitted to the water stress and the
chlorophyll a fluorescence induced by UV light was monitored daily during 16 days. In this work, 40 pots in total, 20 per cultivar were
used in the investigation. Each cultivar was divided in two groups, the control group and the group submitted to the water
stress. The stress response of the cultivars was monitored by red to far-red fluorescence ratio. The data indicate that the
water stress induced the earliest changes on the fluorescence ratio and chlorophyll content for the conventional cultivar.
In addition, a comparative analysis of the fluorescence ratios of the cultivars reveals that conventional plants have higher
chlorophyll content than transgenic ones. This result might be useful in the development of methodologies able to distinguish
conventional to transgenic apart.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0594-4
Authors
A. R. L. Caires, Universidade Federal da Grande Dourados—UFGD Grupo de Óptica Aplicada CP 533 79804–970 Dourados MS Brazil
M. D. Scherer, Universidade Federal da Grande Dourados—UFGD Grupo de Óptica Aplicada CP 533 79804–970 Dourados MS Brazil
T. S. B. Santos, Universidade Federal da Grande Dourados—UFGD Grupo de Óptica Aplicada CP 533 79804–970 Dourados MS Brazil
B. C. A. Pontim, Universidade Federal da Grande Dourados—UFGD Laboratório de Fitopatologia CP 533 79804–970 Dourados MS Brazil
W. L. Gavassoni, Universidade Federal da Grande Dourados—UFGD Laboratório de Fitopatologia CP 533 79804–970 Dourados MS Brazil
S. L. Oliveira, Universidade Federal da Grande Dourados—UFGD Grupo de Óptica Aplicada CP 533 79804–970 Dourados MS Brazil
Abstract The photophysical properties of a series of structurally related 4-aminophthalimides and the corresponding 5-aminophthalic
hydrazides (luminols) are reported. Absorption, steady-state, and time-resolved fluorescence spectra of luminols exhibited
substitution, solvent, and pH dependence. Singlet lifetimes have been determined by time-resolved laser flash spectroscopy.
UV spectra in gas phase and DMSO solution were calculated by TD-DFT which revealed the existence of two low-energy excited
singlet states with strong pH-sensitivity.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-010-0598-0
Authors
Raúl Pérez-Ruiz, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Robert Fichtler, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Yrene Diaz Miara, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Matthieu Nicoul, University of Cologne Department of Physics I Zülpicher Str. 77 50937 Köln Germany
Dominik Schaniel, University of Cologne Department of Physics I Zülpicher Str. 77 50937 Köln Germany
Helfried Neumann, Leibniz-Institute of Catalysis Albert-Einstein-Str. 29a 18059 Rostock Germany
Matthias Beller, Leibniz-Institute of Catalysis Albert-Einstein-Str. 29a 18059 Rostock Germany
Dirk Blunk, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Axel G. Griesbeck, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Axel Jacobi von Wangelin, University of Cologne Department of Chemistry Greinstr. 4 50939 Köln Germany
Abstract Crude petroleum oils are complex mixtures of different compounds (mainly organic), which are obtained from an extensive range
of different geological sources. The fluorescence of crude petroleum oils derives largely from the aromatic hydrocarbon fraction,
and this fluorescence emission is strongly influenced by the chemical composition (e.g., fluorophore and quencher concentrations)
and physical characteristics (e.g., viscosity and optical density) of the oil. The fluorescence spectroscopy (FS) is increasingly
used in petroleum technology due the availability of better optical detection techniques, because FS offers high sensitivity,
good diagnostic potential, and relatively simple instrumentation. In this work we analyzed crude petroleum at different dilution
in Nujol, a transparent mineral oil. The main objective of this work was to verify the possibility to measure crude oil emission
spectroscopic without use of volatile solvents. The mixtures of nujol with different -crude oil concentrations were measured
with a 10 mm optical path cuvette thus simplifying the fluorescence spectroscopy signal detection. The emission spectra were
obtained by exciting the samples with a 400 W Xenon lamp at 350 nm, 450 nm and 532 nm. The emissions of the samples were collected
perpendicularly with the excitation axis.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0586-4
Authors
Juliana Steffens, Polytechnic School, University of São Paulo Department of Chemical Engineering São Paulo Brazil
Eduardo Landulfo, Nuclear and Energetic Research Institute São Paulo Brazil
Lilia Coronato Courrol, Nuclear and Energetic Research Institute São Paulo Brazil
Roberto Guardani, Polytechnic School, University of São Paulo Department of Chemical Engineering São Paulo Brazil
A novel fluorescence enhancement-type derivatizing reagent for amino compounds, 6,7-difluoro-1,4-dihydro-1-methyl-4-oxo-3-quinolinecarboxylic
acid (FMQC), was developed. FMQC reacts with aliphatic primary amino compounds to afford strong fluorescent derivatives having
high photo-and thermo-stabilities. The FMQC derivatives of amino compounds showed 12–159 times higher fluorescence quantum
efficiencies than those of FMQC in aqueous and polar organic media. Additionally, the absorption and fluorescence emission
wavelength of the derivatives are red-shifted from those of FMQC. These differences in the fluorescence properties between
FMQC and the fluorescent derivative enabled the simple and highly sensitive determination of amino compounds without removing
any excess unreacted FMQC by using a simple spectrofluorometer as well as HPLC.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0596-2
Authors
Junzo Hirano, Kyushu University Graduate School of Pharmaceutical Sciences 3-1-1 Maidashi Higashi-ku Fukuoka 812-8582 Japan
Kenji Hamase, Kyushu University Graduate School of Pharmaceutical Sciences 3-1-1 Maidashi Higashi-ku Fukuoka 812-8582 Japan
Hiroyuki Miyata, Kyushu University Graduate School of Pharmaceutical Sciences 3-1-1 Maidashi Higashi-ku Fukuoka 812-8582 Japan
Kiyoshi Zaitsu, Kyushu University Graduate School of Pharmaceutical Sciences 3-1-1 Maidashi Higashi-ku Fukuoka 812-8582 Japan
A fluorometric study has been carried out, for the first time, to investigate the reaction of the new generation antidepressant
sertraline (SRT) with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl). In an alkaline buffered medium (pH 8.0), a green fluorescent
product exhibiting maximum fluorescence intensity at 532 nm after excitation at 470 nm was produced. The factors affecting
the reaction were carefully studied and the conditions were optimized. The kinetics of the reaction was investigated, the
stoichiometry of the reaction was determined, and the mechanism was postulated. The activation energy of the reaction was
determined and found to be 27.34 KJ mole-1. Under the optimum reaction conditions, a linear relationship with good correlation coefficient (r = 0.9998, n = 6) was found between the fluorescence intensity of the reaction product and SRT concentrations in the range of 0.3–20.0 µg ml-1. The limit of detection and limit of quantitation were 0.07 and 0.21 µg ml-1, respectively. The intra- and inter-assay precisions were satisfactory; the relative standard deviations did not exceed 2.61%.
The proposed method was successfully applied to the determination of SRT in its pharmaceutical tablets with good accuracy;
the recovery percentages were 96.97–102.23 ± 1.01–1.62%. The results were compared favorably with those of the reported method.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0595-3
Authors
Ashraf M. Mahmoud, Assiut University Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy Assiut 71256 Egypt
Ibrahim A. Darwish, King Saud University Department of Pharmaceutical Chemistry, College of Pharmacy P.O. Box 2457 Riyadh 11451 Saudi Arabia
Nasr Y. Khalil, King Saud University Department of Pharmaceutical Chemistry, College of Pharmacy P.O. Box 2457 Riyadh 11451 Saudi Arabia
Abstract The influence of antiretroviral drug zidovudine treatment during pregnancy on mandible development in newborn rats was studied. The fluorescence of mandibles from 7-, 14-
and 28-days old individuals was measured by means of fiber-optical fluorescence analyzer with 407 nm laser excitation. Obtained
results revealed disturbing effect of maternal zidovudine administration on mandible fluorescence intensity which should decrease with bone development. Small changes in fluorescence
of porphyrin forms are maintaining in the first month of newborns life while the changes observed in 440–585 nm range disappear.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0591-7
Authors
Zofia Krystyna Drzazga, University of Silesia Chełkowski’s Institute of Physics, Department of Medical Physics 40 007 Katowice ul. Uniwersytecka 4 Poland
Karina Maciejewska, University of Silesia Chełkowski’s Institute of Physics, Department of Medical Physics 40 007 Katowice ul. Uniwersytecka 4 Poland
Katarzyna Michalik, University of Silesia Chełkowski’s Institute of Physics, Department of Medical Physics 40 007 Katowice ul. Uniwersytecka 4 Poland
Michał Kaszuba, Institute of Stomatology 40-534 Katowice ul. Łabędzia 2 Poland
Barbara Nowińska, Medical University of Silesia Faculty of Pharmacy, Department of Pharmacology 41 200 Sosnowiec ul. Jagiellońska 4 Poland
The photoluminescence (PL) spectrum of water-soluble thiol-capped CdTe quantum dots (QDs) conjugated with Tat peptide in solution
showed a remarkable redshift as compared to that of unconjugated QDs. After cellular uptake of the Tat-QDs conjugates, the
micro-PL spectrum of Tat-QDs in lysosomes showed a spectral blueshift, which was most probably due to the fact that Tat peptide
was digested by the enzymes, leaving the Tat-detached QDs in lysosomes. The reasons for the spectral changes have been discussed
in detail.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0579-3
Authors
Rongling Xiong, Fudan University Department of Optical Science and Engineering Shanghai 200433 China
Zheng Li, Fudan University Department of Optical Science and Engineering Shanghai 200433 China
Lan Mi, Fudan University Department of Optical Science and Engineering Shanghai 200433 China
Pei-Nan Wang, Fudan University Department of Optical Science and Engineering Shanghai 200433 China
Ji-Yao Chen, Fudan University Surface Physics Laboratory (National key laboratory), Department of Physics Shanghai 200433 China
Lixin Wang, Fudan University Department of Macromolecular Science and Key Laboratory of Molecular Engineering of Polymers Shanghai 200433 China
Wu-Li Yang, Fudan University Department of Macromolecular Science and Key Laboratory of Molecular Engineering of Polymers Shanghai 200433 China
Novel fluorescing dyes 1,3,4-triphenyl-6-(1,4,7,10-tetraoxa-13-aza-cyclopentadec-13-ylmethyl)-1H-pyrazolo[3,4-b]quinoline (K1) and 2-[(2-hydroxyethyl)-(1,3,4-triphenyl-1H-pyrazolo[3,4-b]quinolin-6-ylmethyl)-amino]ethanol (L1) have been synthesized and investigated by the means of steady state and time-resolved fluorescence techniques. These compounds
act as sensors for the fluorescence detection of small inorganic cations (lithium, sodium, barium, magnesium and calcium)
in solvents of different polarities (THF and acetonitrile). The mechanism, which allows application of these compounds as
sensors, is an electron transfer from the electro-donative part of molecule to the acceptor part (fluorophore), which is retarded
upon complexation of the electro-donative part by inorganic cations. We found that crown ether-containing compound is very
sensitive to the addition of any investigated ions but amino alcohol-containing one exhibits better selectivity to the addition
of two-valued cations. Two kinds of the complexes (LM+ and L2M+) were found in the investigated systems. In addition, the dyes may be used as fluorescence indicators in solvents of lower
polarity like tetrahydrofuran.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0576-6
Authors
Marek Mac, Jagiellonian University Faculty of Chemistry Ingardena 3 30-060 Krakow Poland
Tomasz Uchacz, Jagiellonian University Faculty of Chemistry Ingardena 3 30-060 Krakow Poland
Tomasz Wróbel, Jagiellonian University Student of Faculty of Chemistry Krakow Poland
Andrzej Danel, University of Agriculture Department of Chemistry Balicka 122 31-149 Kraków Poland
Ewa Kulig, University of Agriculture Department of Chemistry Balicka 122 31-149 Kraków Poland
Abstract This work presents spectroscopic properties of some Endocrine Disrupting Compounds (EDCs), frequently found in food and in
natural water. Studied molecules belong to the groups of phenolic and phthalate EDCs. In a first part, we have examined their
absorption and fluorescence properties. Fluorescence emission wavelengths are about 300 nm for phenolic compounds and 360 nm
for phtalate compounds; main excitation wavelengths being comprised between 210 nm and 230 nm. Fluorescence lifetimes measured
are short (about 4 ns) and the fluorescence quantum yield has been determined. In a second part, to avoid the time consuming
solvent extraction step, an analytical application to evaluate the performance of a direct analysis by laser induced fluorescence
spectroscopy of ECDs traces in tap water and in raw water is presented. Good detection limits have been obtained, i.e.: 0.35 µg.L−1 of chlorophenol in tap water, which are always lower than the reported Predictive Non Efficient Concentration (PNEC).
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0583-7
Authors
Badr Benmansour, Université Européenne de Bretagne Brest University, UMR CNRS 6521 CEMCA 6 av. Le Gorgeu 29285 Brest cedex 3 France
Ludovic Stephan, Université Européenne de Bretagne Brest University, UMR CNRS 6521 CEMCA 6 av. Le Gorgeu 29285 Brest cedex 3 France
Jean-Yves Cabon, Université Européenne de Bretagne Brest University, UMR CNRS 6521 CEMCA 6 av. Le Gorgeu 29285 Brest cedex 3 France
Laure Deschamps, Université Européenne de Bretagne Brest University, UMR CNRS 6521 CEMCA 6 av. Le Gorgeu 29285 Brest cedex 3 France
Philippe Giamarchi, Université Européenne de Bretagne Brest University, UMR CNRS 6521 CEMCA 6 av. Le Gorgeu 29285 Brest cedex 3 France
Abstract Polyacrylamide (PAAm) doped by κ-carrageenan (κC) gels were prepared with various amounts of κC varying in the range between
0 wt.% and 3 wt.%. Steady-state fluorescence (SSF) technique was employed for studying sol-gel transition and swelling of
PAAm-κC composite gels which were prepared by free-radical crosslinking copolymerization. Pyranine was introduced as a fluorescence
probe. Pyranine molecules start to bind to acrylamide polymer chains upon the initiation of the polymerization, thus the spectra
of the bonded pyranines shift to the shorter wavelengths. Fluorescence spectra from the bonded pyranines allow one to monitor
the sol-gel transition and to test the universality of the sol-gel transition as a function of some kinetic parameters like
polymer concentration. Observations around the gel point, tc for PAAm-κC composite gels showed that the gel fraction exponent β obeyed the percolation result for low κC (<2.0 wt. %) however classical results were produced at higher κC (>2.0 wt.%). On
the other hand, fluorescence intensity of pyranine was measured during in situ swelling process at various amounts of κC and
it was observed that fluorescence intensity values decreased as swelling is proceeded. Li-Tanaka equation was used to determine
the swelling time constants, τ and cooperative diffusion coefficients, D.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0588-2
Authors
Gülşen Akin Evingur, Istanbul Technical University Faculty of Science and Letters, Physics Department 34469 Maslak- Istanbul Turkey
Önder Pekcan, Kadir Has University 34320 Cibali- Istanbul Turkey
The lymphatic system is essential in oncology and immunology, and in vivo fluorescence imaging plays a major role in assessing
the lymphatic drainage. We investigated non–invasive fluorescence lymph node mapping in mice with special reference to the
assessment of deep abdominal lymph nodes. Quantum dots were injected subcutaneously into the rear footpads of mice, and the
time course of the fluorescent signal was assessed. Visualization of abdominal lymph nodes was compared with and without compression
of the abdomen with transparent, colorless tape at injection doses of 1, 5, and 20 pmol. Popliteal, sacral, iliac, and renal
lymph nodes were delineated by non–invasive imaging. Lymph node signals increased up to 3 h after injection. Compression of
the abdomen markedly improved the visualization of the iliac nodes, which were invisible at 5 pmol without compression and
visible at 1 pmol with compression. Fluorescence lymph node mapping using quantum dots allowed the visualization of deep abdominal
lymph nodes in addition to superficial nodes in intact mice, with the aid of a simple compression technique.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0593-5
Authors
Yusuke Inoue, University of Tokyo Department of Radiology, Institute of Medical Science 4-6-1 Shirokanedai Minato-ku Tokyo 108-8639 Japan
Shigeru Kiryu, University of Tokyo Department of Radiology, Institute of Medical Science 4-6-1 Shirokanedai Minato-ku Tokyo 108-8639 Japan
Makoto Watanabe, University of Tokyo Department of Radiology, Institute of Medical Science 4-6-1 Shirokanedai Minato-ku Tokyo 108-8639 Japan
Naoki Oyaizu, University of Tokyo Department of Laboratory Medicine, Institute of Medical Science Tokyo Japan
Kuni Ohtomo, University of Tokyo Department of Radiology, Graduate School of Medicine Tokyo Japan
Abstract Autofluorescence of the mandible and femur bones taken from newborn rats (7-, 14- and 28-day old) was studied. Endogenous
fluorophores were excited with 231 nm, 291 nm, 340 nm and 360 nm wavelengths. Modifications in content and microenvironment
of both noncolagenous and collagenous constituents of bone tissue as well as metabolic coenzymes during the bone formation
with age were reflected in fluorescence emission spectra. The increase of emission from peptide bonds and tryptophan residues
was noted with rat age while for collagen and metabolic coenzymes at the first 2 weeks only. After maternal administration
of indinavir the changes in fluorescence intensity and shifts in position of peak maximum were found. The distinct drop of
emission from peptide bonds and tryptophan residues in studied bones was detected. In the case of collagen and metabolic coenzymes
the red shift of peak maximum was revealed. Fluorescence spectroscopy could be used to follow bone development in newborn
rats and effect of maternal indinavir administration on offspring.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0584-6
Authors
Zofia Krystyna Drzazga, University of Silesia A. Chełkowski’ Institute of Physics, Department of Medical Physics Uniwersytecka 4 40-007 Katowice Poland
Aneta Kluczewska-Gałka, University of Silesia A. Chełkowski’ Institute of Physics, Department of Medical Physics Uniwersytecka 4 40-007 Katowice Poland
Anna Michnik, University of Silesia A. Chełkowski’ Institute of Physics, Department of Medical Physics Uniwersytecka 4 40-007 Katowice Poland
Michał Kaszuba, Institute of Stomatology Łabędzia 2 40-534 Katowice Poland
Hanna Trzeciak, Medical University of Silesia Department of Prosthetic Dentistry pl. Akademicki 17 41-902 Bytom Poland
The interactions between uranium and four metalloproteins (Apo-HTf, HSA, MT and Apo-EqSF) were investigated using fluorescence
quenching measurements. The combined use of a microplate spectrofluorometer and logarithmic additions of uranium into protein
solutions allowed us to define the fluorescence quenching over a wide range of [U]/[Pi] ratios (from 0.05 to 1150) at physiologically
relevant conditions of pH. Results showed that fluorescence from the four metalloproteins was quenched by UO22+. Stoichiometry reactions, fluorescence quenching mechanisms and complexing properties of metalloproteins, i.e. binding constants and binding sites densities, were determined using classic fluorescence quenching methods and curve-fitting
software (PROSECE). It was demonstrated that in our test conditions, the metalloprotein complexation by uranium could be simulated
by two specific sites (L1 and L2). Results showed that the U(VI)–Apo-HTf complexation constant values (log K1 = 7.7, log K2 = 4.6) were slightly higher than those observed for U(VI)–HSA complex (log K1 = 6.1, log K2 = 4.8), U(VI)–MT complex (log K1 = 6.5, log K2 = 5.6) and U(VI)–Apo-EqsF complex (log K1 = 5.3, log K2 = 3.9). PROSECE fitting studies also showed that the complexing capacities of each protein were different: 550 moles of U(VI)
are complexed by Apo-EqSF while only 28, 10 and 5 moles of U(VI) are complexed by Apo-HTf, HSA and MT, respectively.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0587-3
Authors
Jérôme Michon, Institut de Radioprotection et Sûreté Nucléaire Laboratoire de Radioécologie et Ecotoxicologie Cadarache, Bât 186, BP3 13115 Saint-Paul-lez-Durance Cedex France
Sandrine Frelon, Institut de Radioprotection et Sûreté Nucléaire Laboratoire de Radioécologie et Ecotoxicologie Cadarache, Bât 186, BP3 13115 Saint-Paul-lez-Durance Cedex France
Cédric Garnier, Université Bordeaux I Groupe de Physico Toxico Chimie des Systèmes Naturels, Institut des Sciences Moléculaires, (ISM–UMR CNRS 5255) 33405 Talence Cedex France
Frédéric Coppin, Institut de Radioprotection et Sûreté Nucléaire Laboratoire de Radioécologie et Ecotoxicologie Cadarache, Bât 186, BP3 13115 Saint-Paul-lez-Durance Cedex France
Abstract We tested a Maximum Entropy Method developed for oversampled data (SVD-MEM) on complex analytically simulated exponential
decay data consisting of both noisy and noiseless multi-exponential fluorescence decay curves. We observed recovery of simulated
parameters for three sets of data: a decay containing three exponential functions in both intensity and anisotropy curves,
a set of intensity decays composed of 4, 5 and 6 exponential functions, and a decay characterized by a Gaussian lifetime distribution.
The SVD-MEM fitting of the noiseless data returned the simulated parameters with the high accuracy. Noise added to the data
affected recovery of the parameters in dependence on a data complexity. At selected realistic noise levels we obtained a good
recovery of simulated parameters for all tested data sets. Decay parameters recovered from decays containing discrete lifetime
components were almost independent of the value of the entropy scaling parameter γ used in the maximization procedure when
it changed across the main peak of its posterior probability. A correct recovery of the Gaussian shaped lifetime distribution
required selection of the γ-factor which was by several orders of magnitude larger than its most probable value to avoid a
band splitting.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0589-1
Authors
Jaroslav Vecer, Charles University Faculty of Mathematics and Physics, Institute of Physics Ke Karlovu 5 121 16 Prague 2 Czech Republic
Petr Herman, Charles University Faculty of Mathematics and Physics, Institute of Physics Ke Karlovu 5 121 16 Prague 2 Czech Republic
Steady state and time resolved fluorescence quenching behaviors of meso-Tetrakis (pentafluorophenyl) porphyrin (H2F20TPP) in presence of different aliphatic and aromatic amines have been executed in homogeneous dichloromethane (DCM) solution.
At room temperature in DCM, free base (H2F20TPP) shows fluorescence with two distinct peaks at 640 and 711 nm and natural lifetime τf = 9.8 ns which are very similar to that of meso-tetraphenyl porphyrin (TPP). Unlike TPP, addition of both aliphatic and aromatic
amines to a solution containing H2F20TPP results in an efficient decrease in fluorescence intensity without altering the shape and peak position of fluorescence
emission. Upon addition of amines there was no change in optical absorption spectra of H2F20TPP. The fluorescence quenching rate constants ranged from 1 × 109 to 4 × 109 s−1, which are one order below to the diffusion control limit, and temperature dependent quenching rate constants yield the activation
energies which are found to be order of 0.1 eV. Femto second transient absorption studies reveal the existence of amine cation
radical and porphyrin anion radicals with very short decay time (15 ps). The fluorescence quenching reaction follows Stern–Volmer
kinetics. Steady state and time-resolved data are interpreted within general kinetic scheme of Marcus semi-classical model
which attributes bimolecular electron transfer process between amines and the lowest excited singlet state of H2F20TPP. Calculated internal reorganization energies are found to be in between 0.04 and 0.22 ev. Variation of electron transfer
rate as function of free energy change (∆G0) points the ET reactions in the present systems are in Marcus normal region. This is the first example of reductive fluorescence
quenching of free base neutral porphyrins in homogeneous organic solvent ever known.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s10895-009-0582-8
Authors
Suthari Prashanthi, Indian Institute of Chemical Technology Inorganic and Physical Chemistry Division Institution Uppal Road, Tarnaka Hyderabad 500607 India
P. Hemant Kumar, Indian Institute of Chemical Technology Inorganic and Physical Chemistry Division Institution Uppal Road, Tarnaka Hyderabad 500607 India
Li Wang, Kwansei Gakuin University Department of Chemistry, School of Science 2-1 Gakuen Sanda 669-1337 Japan
Arun Kumar Perepogu, Indian Institute of Chemical Technology Organic Chemistry Division-II Uppal Road, Tarnaka Hyderabad 500607 India
Prakriti Ranjan Bangal, Indian Institute of Chemical Technology Inorganic and Physical Chemistry Division Institution Uppal Road, Tarnaka Hyderabad 500607 India
