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Journal of Fluorescence - published by
Springer -
... is an international forum for peer-reviewed original articles that advance the practice of this established spectroscopic technique.
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Four novel copper(II) complexes of the composition [CuLX] where L = 2,6-bis(benzimidazole-2yl)pyridine, X = dipyridophenazine
(L1), 1,10-phenanthroline (L2), hydroxyproline (L3) and 2,6-pyridine dicarboxylic acid (L4) were synthesized and characterized by using elemental analysis, FT-IR, UV–vis, ESI-MS, molar conductance and magnetic susceptibility
measurements. The complexes [CuLL1](NO3)2 [1], [CuLL2](NO3)2 [2], [CuLL3](NO3) [3] and [CuLL4] (NO3) [4] are stable at room temperature. In DMSO the complexes [1] and [2] are 1:2 electrolytes, [3] and [4] are 1:1 electrolytes. Based on elemental and spectral studies five coordinated geometry is assigned to all the four complexes.
The interaction of four copper ion complexes with calf thymus DNA were carried out by UV–vis titrations, fluorescence spectroscopy,
thermal melting and viscosity measurements .The binding constant (Kb) of the above four metal complexes were determined as 5.43 × 104 M,−1 2.56 × 104 M−1, 1.21 × 104 M−1 and 1.57 × 104 M−1 respectively. Quenching studies of the four complexes indicates that these complexes strongly bind to DNA, out of all complex
1 is binding more strongly. Viscosity measurements indicate the binding mode of complexes with CT DNA by intercalation through
groove. Thermal melting studies also support intercalative binding. The nuclease activity of the above metal complexes shows
that 1, 2 and 3 complexes cleave DNA through redox chemistry.
Content Type Journal Article
Category Original Paper
Pages 1-10
DOI 10.1007/s10895-012-1038-0
Authors
M. Sunita, Department of Chemistry, Osmania University, Hyderabad, 500 007 Andhra Pradesh, India
M. Padmaja, Department of Chemistry, Osmania University, Hyderabad, 500 007 Andhra Pradesh, India
B. Anupama, Department of Chemistry, Osmania University, Hyderabad, 500 007 Andhra Pradesh, India
C. Gyana Kumari, Department of Chemistry, Osmania University, Hyderabad, 500 007 Andhra Pradesh, India
We found that the fluorescence intensity of curcumin (CU) can be highly enhanced by protein bovine serum albumin (BSA) and
human serum albumin (HSA) in the presence of chitosan (CTS). Based on this finding, a new fluorimetric method to determine
the concentration of protein was developed. Under optimized conditions, the enhanced intensities of fluorescence are quantitatively
in proportion to the concentrations of protein in range of 0.007–100 μg·mL−1 for BSA and 0.004–100 μg·mL−1 for HSA at 426 nm excitation, and 0.007–100 μg·mL−1 for BSA and 0.01–100 μg·mL−1for HSA at 280 nm excitation, while corresponding qualitative detection limits (S/N = 3) can lower to 3.96, 2.46, 4.56, 9.20 ng·mL−1, respectively. The method has been successfully used for the determination of HSA in real samples. Based on resonance light
scattering and UV-visible absorption spectroscopic analysis, mechanism studies suggested that the highly enhanced fluorescence
of CU was resulted from synergic effects of favorable hydrophobic microenvironment provided by BSA and CTS and efficient intermolecular
energy transfer between BSA and CU. Protein BSA may bind to CTS through hydrogen bonds, which causes the protein conformation
to convert from β-fold to α-helix. CU can combine with the BSA-CTS complex through its center carbonyl carbon, and CTS plays
a key role in promoting the energy transfer process by shortening the distance between BSA and CU.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0997-x
Authors
Feng Wang, Department of Chemistry and Chemical Engineering, Zaozhuang University, Zaozhuang, 277160 Peoples Republic of China
Wei Huang, Department of Chemistry and Chemical Engineering, Zaozhuang University, Zaozhuang, 277160 Peoples Republic of China
Lingyan Jiang, Department of Chemistry and Chemical Engineering, Zaozhuang University, Zaozhuang, 277160 Peoples Republic of China
Bo Tang, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan, 250014 Peoples Republic of China
In the present study we introduce a Whole-Object Fluorescence Life Time (wo-FLT) measurement approach for ease and a relatively
inexpensive method of tracing alterations in intracellular fluorophore distribution and in the physical-chemical features
of the microenvironments hosting the fluorophore. Two common fluorophores, Rhodamine 123 and Acridine Orange, were used to
stain U937 cells which were incubated, with and without either Carbonyl cyanide 3-chlorphenylhydrazon or the apoptosis inducer
H2O2. The wo-FLT, which is a non-imaging quantitative measurement, was able to detect several fluorescence decay components and
corresponding weights in a single cell resolution. Following cell treatment, both decay time and weight were altered. Results
suggest that the prominent factor responsible for these alterations and in some cases to a shift in emission spectrum as well,
is the intracellular fluorophore local concentration. In this study it was demonstrated that the proposed wo-FLT method is
superior to color fluorescence based imaging in cases where the emission spectrum of a fluorophore remains unchanged during
the investigated process. The proposed wo-FLT approach may be of particular importance when direct imaging is impossible.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1025-x
Authors
Yaniv Namer, The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan, 52900 Israel
Lior Turgeman, Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, 52900 Israel
Mordechai Deutsch, The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and the Technology of the Cellome, Physics Department, Bar-Ilan University, Ramat-Gan, 52900 Israel
Dror Fixler, Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, 52900 Israel
In this report, we show interesting correlation between solvent polarity of alcohol solutions and fluorescence lifetime (τav), estimated from time-resolved fluorescence spectroscopy (TRFS), and macroscopic viscosity (η). Non-functionalized carbon
nanoparticles (NCNP) were successfully used as flurophores in these measurements. The solvent polarity, described through
polarizability (Δf) of dielectric continuum theory, could universally describe both τav/τmax and η/ηmax through the relation,
X(Df) = aOH + b(Df - DfOH) + c(Df - DfOH)2
with X = τav/τmax or η/ηmax, (subscript OH represents corresponding values for alcohols) for alcohol solutions of methanol, ethanol and 1-propanol at
room temperature. We show that fluorescence lifetime and solvent viscosity are universal functions of solvent polarity for
alcohol solutions.
Content Type Journal Article
Category Original Paper
Pages 1-6
DOI 10.1007/s10895-011-1023-z
Authors
Pradip Kumar, Nanomaterials and Nanocomposites Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067 India
H. B. Bohidar, Nanomaterials and Nanocomposites Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067 India
A novel fluorescent Ag+ sensor was developed based on the label-free silver (I) specific oligonucleotide (SSO) and Thioflavine T (ThT) monomer-excimer
switch. C-rich SSO which contain C-C mismatched base pairs can selectively bind to Ag+ ions and the formed duplexes which constructed by C-Ag+-C structure are thermally stabilized without largely altering the double helical structure. ThT give very weak fluorescent
in bulk solution and/or in the presence of SSO. However ThT shows high fluorescence in the presence of SSO and Ag+ at the same time mainly because ThT excimer, which has the high quantum yield, formed and stabilized in the minor or major
groove. Based on the discovery, we developed the novel Ag+ sensor. Under the optimum condition, the selectivity of this system for Ag+ over other metal ions in aqueous solution is remarkably high, and Ag+ can be quantified over the dynamic range of 30–450 nM, with a limit of detection of ~16 nM and a linear correlation coefficient
of 0.995.
Content Type Journal Article
Category Original Paper
Pages 1-5
DOI 10.1007/s10895-011-1031-z
Authors
Yongxiang Wang, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
Fenghua Geng, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
Huiying Xu, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
Peng Qu, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
Xintao Zhou, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
Maotian Xu, Henan Key Laboratory Cultivation Base of Nanobiological Analytical Chemistry, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, 476000 China
In this work, we report the preparation and characterization of novel dye doped fluorophore Eosin yellowish silica nanoparticles
(ESNPs). We synthesized ESNPs by the Stöber method via encapsulation of Eosin Yellowish in silica particles by the condensation
of tetraethyl orthosilicate under alkaline condition at room temperature. The resulted ESNPs were characterized by transmission
electron microscopy, atomic force microscopy; UV–Visible, fluorescence and Fourier transform infrared spectroscopy. The sizes
of the nanoparticles have been found to be 300.0 (±1.0), 400.0 (±1.1) and 500.0 (±5.2) nm depending the reaction conditions
under which they were synthesized. Furthermore, because of intense light emission, the ESNPs were used as fluorophore in a
peroxyoxalate chemiluminescence system. The effect of solvent and concentrations of necessary reagents, bis(2,4,6-trichlorophenyl)oxalate,
sodium salicylate, hydrogen peroxide and the effects of size of the ESNP and temperature on the luminescence efficiency of
the system were examined. The activation kinetic parameters of the system were also evaluated from the temperature investigation.
Content Type Journal Article
Category Original Paper
Pages 1-10
DOI 10.1007/s10895-012-1037-1
Authors
Abdollah Yari, Department of Chemistry, Faculty of Science, Lorestan University, 68137-17133 Khorramabad, Iran
Marzieh Saidikhah, Department of Chemistry, Faculty of Science, Lorestan University, 68137-17133 Khorramabad, Iran
Cancer stands as a leading cause of mortality worldwide and diagnostics of cancer still faces drawbacks. Optical imaging of
cancer would allow early diagnosis, evaluation of disease progression and therapy efficiency. To that aim, we have developed
highly biocompatible PEG functionalized cadmium chalcogenide based three differently luminescent quantum dots (QDs) (CdS,
CdSe and CdTe). Folate targeting scheme was utilized for targeting cancer cell line, MCF-7. We demonstrate the biocompatibility,
specificity and efficiency of our nanotool in detection of cancer cells sparing normal cell lines with retained fluorescence
of functionalized QDs as parental counterpart. This is the first time report of utilizing three differently fluorescent QDs
and we have detailed about the internalization of these materials and time dependent saturation of targeting schemes. We present
here the success of utilizing our biocompatible imaging tool for early diagnosis of cancer.
Content Type Journal Article
Category Original Paper
Pages 1-14
DOI 10.1007/s10895-011-1032-y
Authors
Aby Cheruvathoor Poulose, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Srivani Veeranarayanan, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
M. Sheikh Mohamed, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Sreejith Raveendran, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Yutaka Nagaoka, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Yasuhiko Yoshida, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Toru Maekawa, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
D. Sakthi Kumar, Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Developing of new generation optical polymeric pH sensors has increasing importance due to their stable structures. Available
polymeric sensors for different pH ranges are already needed. In the present study new kinds of monomeric and polymeric absorption
pH sensors (HBANP and PHBANP) derived from 2-amino-4-nitrophenol were prepared. The novel sensors were investigated in various
pH values using spectrophotometric, spectrofluorometric, and electrochemical techniques. The sensors showed sigmoidal correlations
vs. pH range in optical measurements. These correlations were improved as mathematical formula to obtain the solution pH.
HBANP and PHBANP differed from each other by response fields. HBANP had a sharp absorption increase between the pH of 6.5→7.5
while PHBANP spectrophotometrically responded at lower pHs. HBANP was colorless in acidic pHs, yellow-colored in neutral media
and red-colored in alkaline pHs. With its colorimetric responses in various pHs HBANP can be used to develop color-tunable
pH sensors. Electrochemical oxidation peak potentials and currents also particularly changed in various pHs.
Content Type Journal Article
Category Original Paper
Pages 1-10
DOI 10.1007/s10895-011-1034-9
Authors
Mehmet Yıldırım, Faculty of Sciences and Arts, Department of Chemistry, Çanakkale Onsekiz Mart University, TR-17020 Çanakkale, Turkey
Aysel Aydın, Faculty of Sciences and Arts, Department of Chemistry, Çanakkale Onsekiz Mart University, TR-17020 Çanakkale, Turkey
İsmet Kaya, Faculty of Sciences and Arts, Department of Chemistry, Çanakkale Onsekiz Mart University, TR-17020 Çanakkale, Turkey
Detection of Hg2+ in complex natural environmental conditions is extremely challenging, and no entirely successful methods currently exist.
Here we report an easy-to-prepare fluorescent sensor B3 with 2-aminophenol as Hg2+ receptor, which exhibits selective fluorescence enhancement toward Hg2+ over other metal ions. Especially, the fluorescence enhancement was unaffected by anions and cations existing in environment
and organism. Moreover, B3 can detect Hg2+ in sulphide-rich environments without cysteine, S2- or EDTA altering the fluorescence intensity. Consequently, B3 is capable of distinguishing between safe and toxic levels of Hg2+ in more complicated natural water systems with detection limit ≤2 ppb.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-1033-x
Authors
Jiangli Fan, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
Xiaojun Peng, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
Song Wang, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
Xiaojian Liu, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
Honglin Li, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
Shiguo Sun, State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, Dalian, 116024 People’s Republic of China
The potential of novel benzanthrone aminoderivatives to trace the changes in physicochemical properties of lipid bilayer has
been evaluated. Binding of the dyes to the lipid bilayers composed of zwitterionic phospholipid phosphatidylcholine (PC) and
its mixtures with anionic phospholipid cardiolipin (CL) and cholesterol (Chol) was followed by significant quantum yield increase
with small blue shift of emission maximum. Analysis of partition coefficients of the dyes under study showed that all aminobenzanthrones
possess high lipid-associating ability. The dyes A8 and AM2 proved to be sensitive to the variations in membrane chemical
composition responding to the changes in bilayer hydration induced by CL and Chol.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-1035-8
Authors
Valeriya M. Trusova, Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077 Ukraine
Elena Kirilova, Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, 13 Vienibas, Daugavpils, LV5401 Latvia
Inta Kalnina, Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, 13 Vienibas, Daugavpils, LV5401 Latvia
Georgiy Kirilov, Department of Chemistry and Geography, Faculty of Natural Sciences and Mathematics, Daugavpils University, 13 Vienibas, Daugavpils, LV5401 Latvia
Olga A. Zhytniakivska, Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077 Ukraine
Pavel V. Fedorov, Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077 Ukraine
Galyna P. Gorbenko, Department of Biological and Medical Physics, V.N. Karazin Kharkov National University, 4 Svobody Sq., Kharkov, 61077 Ukraine
Poly (ethylene glycol)-co-(L-Lactic acid) diacrylate (PEG-PLLA-DA) copolymers have been extensively investigated for a number of applications in medicine.
PEG-PLLA-DA is biodegradable and the human body can process its degradation products. In this study, we describe the autofluorescence
of PEG-PLLA-DA copolymers and compared it to the fluorescence of poly(ethylene glycol) diacrylate (PEG-DA) and the precursor
molecules used for their synthesis. In addition, we examined the influence of pH on the fluorescence spectra. We found that
PEG-PLLA-DA exhibits higher fluorescence than PEG-DA and all reagents involved in the synthesis of PEG-PLLA-DA. The fluorescence
of PEG-PLLA-DA was affected by pH with fluorescence decreasing at high pH values. At high pH, PEG-PLLA-DA could not polymerize
into hydrogels and exhibited a dramatic decrease in autofluorescence, suggesting that hydrolysis of the ester bond affected
its autofluorescence. At low pH, PEG-PLLA-DA exhibited higher fluorescence and it was able to form crosslinked hydrogels.
The autofluorescence of PEG-PLLA-DA could be exploited to monitor polymer degradation and material structure without the need
to introduce exogenous fluorescent probes. The origin of fluorescence is not clear at this point in time but it appears to
result from a synergetic effect of both lactate units and diacrylate groups in the PEG-PLLA-DA backbone. The observed autofluorescence
of PEG-PLLA-DA persists after reaction of the acrylate groups in the polymerization reaction. This autofluorescence is advantageous
because it could assist in the study of polymers used for drug delivery and tissue engineering applications.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-1029-6
Authors
Yu-Chieh Chiu, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA
Eric M. Brey, Department of Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA
Víctor H. Pérez-Luna, Department of Chemical and Biological Engineering, Illinois institute of Technology, 10 West 33rd Street, Chicago, IL 60616, USA
A library of 92 DNA aptamer sequences was developed against Bacillus anthracis (nonpathogenic Sterne strain) spores and anthrose sugar immobilized on magnetic beads. The selected DNA sequences were studied
for similarities and potential binding pockets between the B. anthracis spore and anthrose aptamers. Several recurring loop structures were identified and tested for their potential to act as aptamer
beacons when labeled with TYE 665 dye on their 5′ ends and Iowa Black quencher on their 3′ ends. Of these candidate sequences,
two beacons designated BAS-6F and BAS-6R emerged which gave strong fluorescence responses at high spore concentrations (greater
than 30,000 spores/ml). These aptamer beacons also detect B. cereus and B. thuringiensis spores with greater fluorescence intensity, but do not strongly detect vegetative cells from an array of other bacterial
species. BAS-6F and 6R are also not capable of detecting pure anthrose, thereby probably ruling that epitope out as a spore
surface target for these particular beacons. While not extremely sensitive, the BAS-6F and 6R aptamer beacons are potentially
valuable for rapid presumptive detection of anthrax or Bacillus spores in suspect powders or bioterrorist activity where spore concentrations are anticipated to be high. The sequence similarities
of these beacons to other published Bacillus spore aptamers are also discussed.
Content Type Journal Article
Category Original Paper
Pages 1-10
DOI 10.1007/s10895-011-1030-0
Authors
John G. Bruno, Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229, USA
Maria P. Carrillo, Operational Technologies Corporation, 4100 NW Loop 410, Suite 230, San Antonio, TX 78229, USA
A simple and sensitive chemiluminescence (CL) method coupled with flow-injection technique is proposed to determine naproxen
(NAP). The method is based upon the enhancement of the weak CL signal arising from the reaction of Ce(IV) and Na2S2O4 with Eu3+ to form the Eu3+-Ce(IV)-Na2S2O4 system. The CL intensity was significantly increased by the introduction of NAP into this system in the presence of silver
nanoparticles (Ag NPs). Examination of the recorded UV–vis spectra and fluorescence spectra indicated that the energy of the
intermediate SO2*, which originated from the redox reaction of Ce(IV) and Na2S2O4, was transferred to Eu3+ via NAP and that the process was accelerated by Ag NPs due to their catalytic activity. Under the optimum conditions, the
CL intensity was increased with increasing NAP concentration and the correlation was linear (r = 0.9992) over the NAP concentration range of 1–420 ng mL−1. The limit of detection (LOD) was 0.11 ng mL−1 with a relative standard deviation (RSD) of 1.15% for 5 replicate determinations of 200 ng mL−1 NAP. The method was successfully applied to determine NAP in pharmaceutical and biological samples.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1026-9
Authors
Mohammad Kamruzzaman, Department of Chemistry, Kyungpook National University, Daegu, 702-701 South Korea
Al-Mahmnur Alam, Department of Chemistry, Kyungpook National University, Daegu, 702-701 South Korea
Kyung Min Kim, Department of Chemistry, Kyungpook National University, Daegu, 702-701 South Korea
Sang Hak Lee, Department of Chemistry, Kyungpook National University, Daegu, 702-701 South Korea
Young Ho Kim, Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu, 702-701 South Korea
Sung Hong Kim, Korea Basic Science Institute Daegu Center, Daegu, 702-701 South Korea
A solution combustion route for the synthesis of Eu3+-activated M2V2O7 (M = Sr, Ba) and their luminescent properties have been investigated. Structure and luminescent characteristics of Sr2V2O7:Eu3+ and Ba2V2O7:Eu3+ nanophosphors have been studied by x-ray diffraction, scanning electron microscopy, transmission electron microscopy, fluorescence
spectrometry and Fourier transform infra-red spectroscopy. The incorporation of Eu3+ activator in these nanoparticles has been checked by luminescence characteristics. These nanoparticles have displayed red
color under a UV source which is due to characteristics transition of Eu3+ from 5D0 → 7F2 at 613 nm in both Sr2V2O7:Eu3+ and Ba2V2O7:Eu3+ nanophosphors. In addition, the optimal Eu3+ - doped contents of Sr2(1-x)Eu2xV2O7 and Ba2(1-x)Eu2xV2O7 nanophosphors for both were 4 mol%.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-1027-8
Authors
Sheetal, Department of Chemistry, Maharshi Dayanand University, Rohtak, 124001 India
V. B. Taxak, Department of Chemistry, Maharshi Dayanand University, Rohtak, 124001 India
S. P. Khatkar, Department of Chemistry, Maharshi Dayanand University, Rohtak, 124001 India
YVO4:Eu3+ phosphors have been prepared by the hydrolytic sol–gel methodology, with and without alkaline catalyst. The solid powder
was obtained by reaction between yttrium III chloride and vanadium alkoxides; the europium III chloride was used as structural
probe. The powder was treated at 100, 400, 600, or 800 °C for 4 h. The samples were characterized by X-ray diffraction, thermal
analysis, and photoluminescence. The XRD patterns revealed YVO4 crystalline phase formation for the sample prepared without the catalyst and heat-treated at 600 °C and for the sample prepared
in the presence of ammonium as catalyst and heat-treated at 100 °C. The average nanosized crystallites were estimated by the
Scherrer equation. The sample which was produced via alkaline catalysis underwent weight loss in two stages, at 100 and 400 °C,
whereas the sample obtained without catalyst presented four stages of weight loss, at 150, 250, 400, and 650 °C. The excitation
spectra of the samples treated at different temperatures displayed the charge transfer band (CTB) at 320 nm. PL data of all
the samples revealed the characteristic transition bands arising from the 5D0 → 5FJ (J = 0, 1, 2, 3, and 4) manifolds under maximum excitation at 320, 394, and 466 nm in all cases. The 5D0 → 7 F2 transition often dominates the emission spectra, indicating that the Eu3+ ion occupies a site without inversion center. The long lifetime suggests that the matrix can be applied as phosphors. In
conclusion, the sol–gel methodology is a very efficient approach for the production of phosphors at low temperature.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1028-7
Authors
Michelle Saltarelli, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Priscilla P. Luz, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Marcela G. Matos, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Emerson H. de Faria, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Katia J. Ciuffi, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Paulo S. Calefi, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Lucas A. Rocha, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
Eduardo J. Nassar, Universidade de Franca, Av. Dr. Armando Salles Oliveira, 201 Pq. Universitário, CEP 14.404-600 Franca, SP, Brazil
The chiral recognition phenomenon was observed in enantioselective and excited-state energy transfer processes. Based on bimolecular
luminescence quenching kinetics for a system containing chiral molecules, the quenching efficiency was evaluated by Stern-Volmer
equation for a system containing a chiral R(+) and S(−) resolved quencher species. The utility of this methodology is confirmed
by examining the enantio-selective excited-state quenching between Eu(dpa)3 complex (where dpa = pyridine-2,6-dicarboxylate) acting as the energy donor to R(+)-1,1-Bi(2-Naphthol) and S(−)-1,1-Bi(2-naphthol)
as the energy acceptor was studied in solution. The results of this study confirm the utility of luminescence measurements
as a probe of chiral discriminatory behavior.
Content Type Journal Article
Category Original Paper
Pages 1-4
DOI 10.1007/s10895-011-1024-y
Authors
Esam Bakir, Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
The three Ru(II) complexes of [Ru(phen)2dppca]2+ (1) [Ru(bpy)2dppca]2+ (2) and [Ru(dmb)2dppca]2+ (3) (where phen = 1,10 phenanthroline, bpy = 2,2-bipyridine, dmb = 2 ,2-dimethyl 2′,2′-bipyridine and polypyridyl ligand
containing a single carboxylate functionality dppca ligand (dipyridophenazine-11-carboxylic acid) have been synthesized and
characterized. These complexes have been shown to act as promising calf thymus DNA intercalators and a new class of DNA light
switches, as evidenced by UV-visible and luminescence titrations with Co2+ and EDTA, steady-state emission quenching by [Fe(CN)6]4− and KI, DNA competitive binding with ethidium bromide, viscosity measurements, and DNA melting experiments. The results suggest
that 1, 2, and 3 complexes bind to CT-DNA through intercalation and follows the order 1 > 2 > 3. Under irradiation at 365 nm,
the three complexes have also been found to promote the photocleavage of plasmid pBR322 DNA.
Content Type Journal Article
Category Original Paper
Pages 1-13
DOI 10.1007/s10895-011-1018-9
Authors
Praveen Kumar Yata, Department of Chemistry, Osmania University, Hyderabad, 500007 Andhra Pradesh, India
M. Shilpa, Department of Chemistry, Osmania University, Hyderabad, 500007 Andhra Pradesh, India
P. Nagababu, Institute of Chemistry, Academia Sinica, Nankang, Taipei
M. Rajender Reddy, Department of Chemistry, Osmania University, Hyderabad, 500007 Andhra Pradesh, India
Laxma Reddy Kotha, Instituto de Tecnologia Química e Biológica (ITQB) Universidade Nova de Lisboa Av. da República, EAN 2780-157 Oeiras, Portugal
Nazar Md Gabra, Department of Chemistry, Osmania University, Hyderabad, 500007 Andhra Pradesh, India
S. Satyanarayana, Department of Chemistry, Osmania University, Hyderabad, 500007 Andhra Pradesh, India
A new, simple, sensitive and selective spectrofluorimetric method for the determination of Hydrochlorothiazide was developed
in acetonitrile at pH 6.2. The Hydrochlorothiazide can remarkably enhance the luminescence intensity of the Tb3+ ion doped in sol–gel matrix at λex = 370 nm. The intensity of the emission band of Tb3+ ion doped in sol–gel matrix was increased due to the energy transfer from the triplet excited state of Hydrochlorothiazide
to (5D4) excited energy state of Tb3 ion. The enhancement of the emission band of Tb3+ ion doped in sol–gel matrix at (5D4→7 F5) 545 nm was directly proportion to the concentration of Hydrochlorothiazide with a dynamic ranges of 5.0 × 10−10—5.0 × 10−6 mol L−1 and detection limit of 2.2 × 10−11 mol L−1.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1017-x
Authors
A. O. Youssef, Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
Simple, accurate and sensitive spectrofluorimetric method has been proposed for the determination of three cephalosporins,
namely; cefixime (cefi), cephalexine (ceph), cefotaxime sodium (cefo) in pharmaceutical formulations. The method is based
on a reaction between cephalosporins with 1, 2-naphthoquinone-4-sulfonic (NQS) in alkaline medium, at pH values of 12.0 for
cefi and 13.0 for ceph and cefo to give highly fluorescent derivatives extracted with chloroform and subsequently measured
at 600,580 and 580 nm after excitation at 520,455 and 490 nm for cefi, ceph and cefo respectively. The optimum experimental
conditions have been studied. Beer’s law is obeyed over the concentrations of 10–35 ng/mL, 10–60 ng/mL and 20–45 ng/mL for
cefi,ceph and cefo, respectively. The detection limits were 2.02 ng/mL, 2.09 ng/mL and 2.30 ng/mL for cefi, ceph and cefo,
respectively, with a linear regression correlation coefficient of 0.9987, 0.9995 and 0.9991 and recoveries in range from 98.5-107.04,
95.17-101.00 and 95.00-109.55% for cefi, ceph and cefo, respectively. This method is simple and can be applied for the determination
of cefi, ceph and cefo in pharmaceutical formulations in quality control laboratories.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1021-1
Authors
Abdalla A. Elbashir, Faculty of Science, Chemistry Department, University of Khartoum, Khartoum, Sudan
Shazalia M. Ali Ahmed, Faculty of Science, Chemistry Department, University of Khartoum, Khartoum, Sudan
Hassan Y. Aboul-Enein, Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, Cairo, 12311 Egypt
A new bis(rhodamine)-based fluorescent probe 4 was synthesized, and it exhibited high selectivity for Fe3+ over other commonly coexistent metal ions in both 50% ethanol and Tris–HCl buffer. Upon the addition of Fe3+, the spirocyclic ring of 4 was opened and a significant enhancement of visible color and fluorescence in the range of 500–600 nm was observed.
Content Type Journal Article
Category Rapid Communication
Pages 1-6
DOI 10.1007/s10895-011-1022-0
Authors
Xiaopo Chen, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
Huijie Hong, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
Rui Han, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
Di Zhang, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
Yong Ye, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
Yu-fen Zhao, Phosphorus Chemical Engineering Research Center of Henan Province, Department of Chemistry, Zhengzhou University, Zhengzhou, 450052 China
The absorption and fluorescence spectra of α-carboline, 9H-pyrido[2,3-b]indole, AC, in organic aprotic solvents containing
different water proportions and in acid/base aqueous solutions inside and outside the pH range have been examined. In the
organic aprotic solvents, the addition of increasing concentrations of water sequentially quenches and shifts to the red the
fluorescence spectra of AC. These spectral changes have been rationalized assuming the formation, at the lower water concentrations,
of a discrete ground state non-cyclic weakly fluorescent AC hydrate emitting at 376 nm that, upon increasing the water concentrations,
evolves to a higher order AC poly hydrate emitting at 397 nm. The changes of the AC absorption spectra in aqueous acid/basic
solutions indicate the existence of three ground state prototropic species; the pyridinic protonated cation, C (pKa = 4.10 ± 0.05), the neutral, N (pKa = 14.5 ± 0.2), and the pyrrolic deprotonated anion, A. Conversely, the changes of the AC fluorescence spectra in these media
indicate the existence of four excited state species emitting at 376 nm, 397 nm, 460 nm and 465 nm. Since the emissions at
376 nm and 397 nm satisfactorily match those of the hydrates observed in the organic-water mixtures, they were consistently
assigned to two differently hydrated ground state N species. The remaining emissions at 460 nm and 465 nm have been assigned
without ambiguity, on the basis of their excitation spectra, to the C and A species, respectively. The excited-state pKas of the prototropic species of AC have been estimated by using the Förster-Weller cycle.
Content Type Journal Article
Category Original Paper
Pages 1-11
DOI 10.1007/s10895-011-1016-y
Authors
Emilio García-Fernández, Departamento de Química Física, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Carmen Carmona, Departamento de Química Física, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
María A. Muñoz, Departamento de Química Física, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
José Hidalgo, Departamento de Química Física, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Manuel Balón, Departamento de Química Física, Facultad de Farmacia, Universidad de Sevilla, 41012 Sevilla, Spain
Graphite oxide was characterized by pH dependent excitation-emission matrices from 300 to 500 nm in excitation and from 320
to 600 nm in emission which reveal the presence of two pH steps. These are assigned to the presence of carboxy groups and
phenolic hydroxy groups, respectively. Fluorescence is strongest at 470 nm excitation and 555 nm emission. The fluorescence
intensity is a function of pH but not of temperature, and is not quenched by oxygen.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-1019-8
Authors
Sven Kochmann, Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93053 Regensburg, Germany
Thomas Hirsch, Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93053 Regensburg, Germany
Otto S. Wolfbeis, Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93053 Regensburg, Germany
Based on the frequently-used cyanine dye probe thiazole orange (TO), a novel kind of cyanine dye was designed and synthesized.
Carbazole was inserted into the methylidyne structure of TO as a bridge to afford a kind of novel styryl cyanine dye with
carbazole bridged chain. The dyes were characterized by HNMR and MS. The spectra of the novel dyes were also studied and the
results showed that the fluorescent wavelength of novel carbzole dye shifted red for 100 nm, stock shift increased by 70 nm
and the fluorescent intensity enhanced by 70 times compared to that of TO. When the novel dye was labeled by BSA, its fluorescent
wavelength changed little and the intensity enhanced. It is indicated that it can be used as an excellent fluorescent probe
in biological labeling.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1014-0
Authors
Xuening Fei, School of Chemical Engineering and Technology, TianJin University, Tianjin, 300072 China
Yingchun Gu, School of Chemical Engineering and Technology, TianJin University, Tianjin, 300072 China
Chao Li, Department of Material Science and Engineering, TianJin Institute of Urban Construction, Tianjin, 300384 China
Xu Yang, Department of Material Science and Engineering, TianJin Institute of Urban Construction, Tianjin, 300384 China
CdHgTe/SiO2 nanoparticles were prepared by SiO2 capping on the surface of CdHgTe QDs. The characteristics, such as optical spectra, photostability, size and cell toxicity
were investigated. The dynamic distribution of CdHgTe/SiO2 nanoparticles was in vivo monitored by near infrared fluorescence imaging system. CdHgTe/SiO2 nanoparticles acted as a novel fluorescence probe have a maximum fluorescence emission of 785 nm and high photo-stability.
The hydrodynamic diameter of CdHgTe/SiO2 nanoparticles could be adjusted to 122.3 nm. Compared to CdHgTe QDs, inhibitory effects of CdHgTe/SiO2 nanoparticles on proliferation of HCT116 cells decreased to a certain extent. CdHgTe/SiO2 nanoparticles had their specific dynamic distribution behavior, which provided new perspectives for bio-distribution of nanoparticles.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1005-1
Authors
Haiyan Chen, Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009 China
Sisi Cui, Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009 China
Zhenzhen Tu, Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009 China
Yueqing Gu, Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009 China
Xuemei Chi, Department of Biomedical Engineering, School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Lane, Gulou District, Nanjing, 210009 China
In this paper, enhanced fluorescence from a silver film coated nanosphere templated grating is presented. Initially, numerical
simulation was performed to determine the plasmon resonance wavelength by varying the thickness of the silver film on top
of a monolayer of 400 nm nanospheres. The simulation results are verified experimentally and tested for enhancing fluorescence
from fluorescein isothiocyanate whose excitation wavelength closely matches with the plasmon resonance wavelength of the substrate
with 100 nm silver film over nanosphere. The 12 times enhancement in the intensity is attributed to the local field enhancement
in addition to the excitation of surface plasmon polaritons along the surface.
Content Type Journal Article
Category Original Paper
Pages 1-6
DOI 10.1007/s10895-011-0996-y
Authors
Ravi Kumar Kannadorai, School of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798 Singapore
U. S. Dinish, Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667 Singapore
Chit Yaw Fu, Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667 Singapore
Gopalkrishna Hegde, Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore, 560012 India
Malini Olivo, Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, Singapore, 138667 Singapore
Anand Asundi, School of Mechanical & Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798 Singapore
Using fluorescence probing technology, we studied the mechanism and interrelations of calcium release and H2O2 production in situ in living tissues of tobacco and cotton plants which were induced by pathogen elicitor, salicylic acid
(SA) and pectinase respectively. Results showed that (1) pathogen elicitors could induced H2O2 response in epidermis cells regardless of environmental calcium, but in mesophyll protoplast, H2O2 response could only be induced at calcium condition. Similarly, SA and pectinase induced H2O2 response could only be observed at calcium condition; (2) pathogen elicitors could induce calcium response in both epidermis
cells and protoplasts regardless of environmental calcium, while calcium response couldn’t be induced at non-calcium condition
by SA and pectinase; (3) H2O2 response and calcium response in protoplast were faster than that in the whole cell. These results indicated that pathogen
elicitors can induce the release of cell wall calcium and the cell wall calcium release is independent to pectinase. And it
is concluded that free calcium influx is necessary for the oxidative burst and cell wall calcium has an irreplaceable role
in defense signal transduction.
Content Type Journal Article
Category Original Paper
Pages 1-9
DOI 10.1007/s10895-011-0992-2
Authors
Quan Gan, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People’s Republic of China
Bin Jia, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People’s Republic of China
Xiao Liu, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People’s Republic of China
Yizhu Zhang, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People’s Republic of China
Manxi Liu, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074 People’s Republic of China
Optical imaging with near-infrared (NIR) fluorescent probes is a useful diagnostic technology for in vivo tumor detection. Our plan was to develop novel NIR fluorophore-micelle complex probes. IC7-1 and IC7-2 were synthesized as
novel lipophilic NIR fluorophores, which were encapsulated in an amphiphilic polydepsipeptide micelle “lactosome”. The fluorophore-micelle
complexes IC7-1 lactosome and IC7-2 lactosome were evaluated as NIR fluorescent probes for in vivo tumor imaging. IC7-1 and IC7-2 were synthesized and then encapsulated in lactosomes. The optical properties of IC7-1, IC7-2,
IC7-1 lactosome and IC7-2 lactosome were measured. IC7-1 lactosome and IC7-2 lactosome were administered to tumor-bearing
mice, and fluorescence images were acquired for 48 h. IC7-1 and IC7-2 were successfully synthesized in 12% and 6.3% overall
yield, and maximum emission wavelengths in chloroform were observed at 858 nm and 897 nm, respectively. Aqueous buffered solutions
of IC7-1 lactosome and IC7-2 lactosome showed similar fluorescence spectra in chloroform and higher or comparable quantum
yields and higher photostability compared with ICG. Both lactosome probes specifically visualized tumor tissue 6 h post-administration.
IC7-1 lactosome and IC7-2 lactosome could be promising NIR probes for in vivo tumor imaging.
Content Type Journal Article
Category Original Paper
Pages 1-9
DOI 10.1007/s10895-011-1007-z
Authors
Yoichi Shimizu, Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501 Japan
Takashi Temma, Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501 Japan
Isao Hara, Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto, 619-0237 Japan
Ryo Yamahara, Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto, 619-0237 Japan
Ei-ichi Ozeki, Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Soraku-gun, Seika-cho, Kyoto, 619-0237 Japan
Masahiro Ono, Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501 Japan
Hideo Saji, Department of Patho-Functional Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8501 Japan
The self-assembly of non-toxic well-consumed small caffeine molecules into well-defined structures has important implications
for future medical applications seeking to target the transport of small drugs in human body. Particularly, the solvation
of the microenvironments of the self assembly ultimately dictates the interaction with the drug molecules and their therapeutic
efficacy. We present femtosecond-resolved studies of the dynamics of aqueous solvation within self-assembled dimeric structure
of caffeine molecules. We have placed small hydrophobic probes 4-(dicyanomethylene)-2-methyl-6-(p-dimethylaminostyryl) 4H-pyran
(DCM), coumarin 500 (C500) into the caffeine dimer to enable spectroscopic examinations of the interior. While molecular modeling
and NMR studies of the probes in the caffeine dimers reveal a well-defined location (stacked in between two caffeine molecules),
dynamical light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, densimetric and sonometric experiments explore
the structural evolution of the dimer upon complexation with the probes. We have extended our studies in various temperatures
in order to explore structural evolution of the self assembled structure and consequently the dynamics of solvation in the
interior of the dimer. Picoseconds/femtosecond resolved dynamics and the polarization gated spectroscopic studies unravel
the hydration and energetics associated with activated viscous flow of the confined probes. Our studies indicate that the
interior of the caffeine dimer is well-solvated; however, the dynamics of solvation is retarted significantly compared to
that in bulk water, clearly revealing the dimers maintain some ordered water molecules. We have also explored the consequence
of the retarded dynamics of solvation on the photo-induced electron transfer (ET) reaction of a model probe, 2-(p-toluidino)
naphthalene-6-sulfonate (TNS) encapsulated in the dimer.
Content Type Journal Article
Category Original Paper
Pages 1-17
DOI 10.1007/s10895-011-1011-3
Authors
Soma Banerjee, Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India
Pramod Kumar Verma, Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India
Rajib Kumar Mitra, Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India
Gautam Basu, Department of Biophysics, BOSE INSTITUTE, P 1/12, C. I. T. Road, Scheme – VIIM, Kolkata, 700054 West Bengal, India
Samir Kumar Pal, Unit for Nano Science & Technology, Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata 700 098, India
Some novel Y-shaped imidazole derivatives were developed and characterized by NMR and mass spectral techniques. The photophysical
properties of these imidazole derivatives were studied in several solvents. The Kamlet-Taft and Catalan’s solvent scales were
found to be the most suitable for describing the solvatochromic shifts of the absorption and fluorescence emission. The adjusted
coefficient representing the electron releasing ability or basicity of the solvent, Cβ or CSB has a negative value, suggesting that the absorption and fluorescence bands shift to lower energies with the increasing electron-donating
ability of the solvent. This effect can be interpreted in terms of the stabilization of the resonance structures of the chromophore.
The observed lower fluorescence quantum yield may be due to an increase in the non-radiative deactivation rate constant. This
is attributed to the loss of planarity in the excited state provided by the non co-planarity of the cinnamaldehyde ring attached
to C(2) atom of the imidazole ring. Such a geometrical change in the excited state leads to an important Stokes shift, reducing
the reabsorption and reemission effects in the detected emission in highly concentrated solutions.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1009-x
Authors
J. Jayabharathi, Department of Chemistry, Annamalai University, Annamalainagar, 608 002 Tamilnadu, India
V. Thanikachalam, Department of Chemistry, Annamalai University, Annamalainagar, 608 002 Tamilnadu, India
K. Brindha Devi, Department of Chemistry, Annamalai University, Annamalainagar, 608 002 Tamilnadu, India
M. Venkatesh Perumal, Department of Chemistry, Annamalai University, Annamalainagar, 608 002 Tamilnadu, India
A novel, simple, sensitive and selective spectrofluorimetric method was developed for the determination of trace amounts of
chlorzoxazone and Ibuprofen in pharmaceutical tablets using optical sensor Eu-Tetracycline HCl doped in sol–gel matrix. The
chlorzoxazone or Ibuprofen can remarkably enhance the luminescence intensity of Eu-Tetracycline HCl complex doped in a sol–gel
matrix in dimethylformamide (DMF) at pH 9.7 and 6.3, respectively, λex = 400 nm. The enhancing of luminescence intensity peak of Eu-Tetracycline HCl complex at 617 nm is proportional to the concentration
of chlorzoxazone or Ibuprofen a result that suggested profitable application as a simple optical sensor for chlorzoxazone
or Ibuprofen assessment. The dynamic ranges found for the determination of chlorzoxazone and Ibuprofen concentration are 5 × 10−9–1 × 10−4 and 1 × 10−8–7 × 10−5 mol L−1, and the limit of detection (LOD) and quantitation limit of detection (LOQ) are 3.1 × 10−10 , 9.6 × 10−10 and 5.6 × 10−10, 1.7 × 10−9 mol L−1, respectively.
Content Type Journal Article
Category Original Paper
Pages 1-10
DOI 10.1007/s10895-011-1013-1
Authors
M. S. Attia, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
M. N. Ramsis, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
L. H. Khalil, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
S. G. Hashem, Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt
Eu(III)-9-acridinecarboxylate (9-ACA) complex was synthesized and characterized by elemental analysis, conductivity measurement,
IR spectroscopy, thermal analysis, mass spectroscopy, 1H-NMR, fluorescence and ultraviolet spectra. The results indicated that the composition of this complex is [Eu(III)-(9-ACA)2(NCS)(C2H5OH)2] 2.5 H2O and the oxygen of the carbonyl group coordinated to Eu(III). The interaction between the complex with nucleotides guanosine
5′- monophosphate (5′-GMP), adenosine 5′-diphosphates (5′-ADP), inosine (5′-IMP) and CT-DNA was studied by fluorescence spectroscopy.
The fluorescence intensity of Eu(III)-9-acridinecarboxylate complex was enhanced with the addition of CT-DNA. The effect of
pH values on the fluorescence intensity of Eu(III) complex was investigated. Under experimental conditions, the linear range
was 9–50 ng mL−1 for calf thymus DNA (CT- DNA) and the corresponding detection limit was 5 ng mL−1. The results showed that Eu(III)-(9-ACA)2 complex binds to CT-DNA with stability constant of 2.41 × 104 M .
Content Type Journal Article
Category Original Paper
Pages 1-11
DOI 10.1007/s10895-011-1000-6
Authors
Hassan A. Azab, Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522 Egypt
Belal H. M. Hussein, Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, 41522 Egypt
Abdullah I. El-Falouji, Biotechnology Research Center, Suez Canal University, Ismailia, 41522 Egypt
The development of screening assays continues to be an active area of research in molecular diagnostics. Fluorescent microspheres
conjugated to biomarkers (nucleic acids, proteins, lipids, carbohydrates) and analyzed on flow cytometer instruments offered
a new approach for multiplexed detection platform in a suspension format. Quantum dots encoded into synthetic microspheres
have the potentials to improve current screening bioassays and specifically suspension array technology. In this paper, commercialized
quantum dot-encoded microsphere were evaluated and optimized as fluorescent probes to address some of the limitations of suspension
array technologies. A comprehensive study was undertaken to adapt the bioconjugation procedure to the quantum dot-encoded
microsphere structural and optical properties. Both the leaching-out of quantum dots and microspheres degradation under bioconjugation
experimental conditions were minimized. A rapid, efficient and reproducible conjugation method was developed for the detection
of single-stranded DNA with the commercialized quantum dot-encoded microsphere. Approximately ten thousand microspheres were
conjugated to short amino-modified DNA sequences in one hour with high efficiency. The bioconjugated microspheres acting as
fluorescent probes successfully detected a DNA target in suspension with high specificity. Quantum dot-encoded microsphere
commercial products are limited which strongly prevents reproducible and comparative studies between laboratories. The method
developed here contributes to the understanding of quantum dot-encoded microsphere reactivity, and to the optimization of
adapted experimental procedure. This step is essential in the development of this new fluorescent probe technology for multiplex
genotyping assay and molecular diagnostic applications.
Content Type Journal Article
Category Original Paper
Pages 1-13
DOI 10.1007/s10895-011-1004-2
Authors
Sarah Thiollet, Biosensors and Diagnostic Laboratory, Cranfield Health, Cranfield University, Check-Points B.V., Wageningen, The Netherlands
Séamus Higson, Biosensors and Diagnostic Laboratory, Cranfield Health, Cranfield University, MK43 0AL Cranfield, UK
Nicola White, Translational Medicine Laboratory, Cranfield Health, Cranfield University, MK43 0AL Cranfield, UK
Sarah L. Morgan, Translational Medicine Laboratory, Cranfield Health, Cranfield University, MK43 0AL Cranfield, UK
Salicylaldehyde was found to have a high selectivity for zinc ions with simultaneous enhancement of fluorescence in aqueous
buffer solution at optimum pH 8.5. The stoichiometry of the complex was determined to be 1:1 with a Ka value of 3.4 × 104 M−1 at 298 K. The fluorescence of the complex is not affected by common anions and Zn2+ binds preferentially to salicylaldehyde in the presence of alkali, alkaline earth and heavy metal cations (Hg2+, Cd2+, Cr3+ and Ni2+). This property is not observed with related phenolic compounds bearing a carbonyl group such as esters, amides, carboxylic
acids and ketones.
A simple fluorescent sensor 1 has been developed for the recognition of Fe(III) in semi-aqueous solution at pH 7.0. The sensor, containing two Schiff base
type receptors directly connected to naphthalene fluorophores, shows a concentration dependent decrease in emission intensity
upon Fe(III) addition. The sensor was selective for Fe(III) over other metal ions and can measure Fe(III) ion concentration
between 0.05 and 0.12 mM. The binding stoichiometry was established as 1:1 (host: guest) with a binding constant (Logβ) of
4.01. Furthermore, the addition of Fe(III) to a solution of 1 caused a colour change from light yellow to colourless meaning 1 is also capable of detecting Fe(III) by the naked eye.
Content Type Journal Article
Category Short Communication
Pages 1-4
DOI 10.1007/s10895-011-1015-z
Authors
María José Casanueva Marenco, Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Campus Rıo S. Pedro, 11510 Puerto Real, Cadiz, Spain
Colin Fowley, Department of Pharmacy and Pharmaceutical Sciences, School of Biomedical Sciences, The University of Ulster, Coleraine, Northern Ireland BT52 1SA
Barry W. Hyland, Department of Pharmacy and Pharmaceutical Sciences, School of Biomedical Sciences, The University of Ulster, Coleraine, Northern Ireland BT52 1SA
Dolores Galindo-Riaño, Department of Analytical Chemistry, Faculty of Sciences, University of Cadiz, Campus Rıo S. Pedro, 11510 Puerto Real, Cadiz, Spain
Suban K. Sahoo, Department of Applied Chemistry, Sardar Vallabhbhai National Institute Technology (SVNIT), Surat, 395 007 Gujrat, India
John F. Callan, Department of Pharmacy and Pharmaceutical Sciences, School of Biomedical Sciences, The University of Ulster, Coleraine, Northern Ireland BT52 1SA
The interaction of 2,2-diphenyl-1-picrylhydrazyl (DPPH●) free radical with thiol-capped CdTe quantum dots (QDs) has been studied by UV–vis spectroscopy, steady state and time resolved
fluorescence measurements. Addition of DPPH● radical to CdTe QDs resulted in fluorescence quenching. The interaction occurs through static quenching as this was confirmed
by fluorescence lifetime measurements. Time course absorption studies indicates that DPPH● may be reduced by interaction with QDs to the substituted hydrazine form (2,2-diphenyl-1-picrylhydrazine) DPPH-H. The mechanism
of fluorescence quenching of CdTe QDs by DPPH● is proposed.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1012-2
Authors
Oluwasesan Adegoke, Department of Chemistry, Rhodes University, Grahamstown, 6140 South Africa
Wadzanai Chidawanyika, Department of Chemistry, Rhodes University, Grahamstown, 6140 South Africa
Tebello Nyokong, Department of Chemistry, Rhodes University, Grahamstown, 6140 South Africa
LED induced chlorophyll fluorescence analysis is employed to investigate the effect of water deficit and salt stress upon
the growth process of Jatropha curcas L.. Red(Fr) and far-red(FFr) chlorophyll fluorescence around 685 nm and 735 nm, respectively, were observed and examined
as a function of the stress intensity(salt concentration and water deficit). The fluorescence ratio Fr/FFr which is a valuable
nondestructive and nonintrusive indicator of the chlorophyll content of leaves was exploited to monitor the jatropha plants under stress. The data indicated that salinity plays a minor role in the chlorophyll concentration of leaves for NaCl
concentrations in the 25 to 200 mM range. The fluorescence ratio also permitted the detection of damage caused by water deficit
in the early stages of the plants growing process. A significant variation of the Fr/FFr ratio was observed in the first 10 days
of the experiment, and before signs of visual stress became apparent. The results suggest that the Fr/FFr ratio is an early-warning
indicator of water deficit stress
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0998-9
Authors
E. A. Silva, Departamento de Física, Universidade Federal Rural de Pernambuco, Recife, Brazil
A. S. Gouveia-Neto, Departamento de Física, Universidade Federal Rural de Pernambuco, Recife, Brazil
R. A. Oliveira, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Brazil
D. S. Moura, Departamento de Física, Universidade Federal Rural de Pernambuco, Recife, Brazil
P. C. Cunha, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife, Brazil
E. B. Costa, Departamento de Física, Universidade Federal Rural de Pernambuco, Recife, Brazil
T. J. R. Câmara, Departamento de Química, Universidade Federal Rural de Pernambuco, Recife, Brazil
L. G. Willadino, Departamento de Biologia, Universidade Federal Rural de Pernambuco, Recife, Brazil
This work describes the application of time resolved fluorescence in microtiterplates and electrochemical methods on glassy
carbon electrode for investigating the interactions of europium-3-carboxycoumarin with pesticides aldicarb, methomyl and prometryne.
Stern-volmer studies at different temperatures indicate that static quenching dominates for methomyl, aldicarb and prometryne.
By using Lineweaver-Burk equation binding constants were determined at 303 K, 308 K and 313 K. A thermodynamic analysis showed
that the reaction is spontaneous with ΔG being negative. The enthalpy ΔH and the entropy ΔS of reactions were all determined.
A time-resolved (gated) luminescence-based method for determination of pesticides in microtiterplate format using the long-lived
europium-3-carboxycoumarin has been developed. The limit of detection is 4.80, 5.06 and 8.01 μmol L−1 for methomyl, prometryne and aldicarb, respectively. This is the lowest limit of detection achieved so far for luminescent
lanthanide-based probes for pesticides. The interaction of the probe with the pesticides has been investigated using cyclic
voltammetry (CV), differential pulse polarography (DPP), square wave voltammetry (SWV) and linear sweep voltammetry (LSV)
on a glassy carbon electrode in I = 0.1 mol L−1 p-toluenesulfonate at 25 °C. The diffusion coefficients of the reduced species are calculated. The main properties of the
electrode reaction occurring in a finite diffusion space are the quasireversible maximum and the splitting of the net SWV
peak for Eu(III) ions in the ternary complex formed . It was observed that the increase of the cathodic peak currents using
LSV is linear with the increase of pesticides concentration in the range 5 × 10−7 to 1 × 10−5 mol L−1. The detection limit (DL) were about 1.01, 2.23 and 1.89 μmolL−1 for aldicarb, methomyl and prometryne, respectively. In order to assess the analytical applicability of the method, the influence
of various potentially interfering species was examined. Influence of interfering species on the recovery of 10 μmol L−1 pesticides has been investigated.
Content Type Journal Article
Category Original Paper
Pages 1-18
DOI 10.1007/s10895-011-1002-4
Authors
Hassan A. Azab, Chemistry Department, Faculty of science, Suez Canal University, Ismailia, 41522 Egypt
Axel Duerkop, Institute of Analytical Chemistry, Chemo and Biosensors, Regensburg University, 93040 Regensburg, Germany
E. M. Mogahed, Chemistry Department, Faculty of Science, Suez Canal University, Suez, Egypt
F. K. Awad, Chemistry Department, Faculty of Science, Suez Canal University, Suez, Egypt
R. M. Abd El Aal, Chemistry Department, Faculty of Science, Suez Canal University, Suez, Egypt
Rasha M. Kamel, Chemistry Department, Faculty of Science, Suez Canal University, Suez, Egypt
Europium doped glass-ceramics containing BaF2 nano-crystals have been prepared by using the controlled crystallization of melt-quenched glasses. X-ray diffraction and
transmission electron microscopy have confirmed the presence of cubic BaF2 nano-crystalline phase in glass matrix in the ceramized samples. Incorporation of rare earth ions into the formed crystalline
phase having low phonon energy of 346 cm−1 has been demonstrated from the emission spectra of Eu3+ ions showing the transitions from upper excitation states 5DJ (J = 1, 2, and 3) to ground states for the glass-ceramics samples. The presence of divalent europium ions in glass and glass-ceramics
samples is confirmed from the dominant blue emission corresponding to its 5d-4f transition under an excitation of 300 nm. Increase in the reduction of trivalent europium (Eu3+) ions to divalent (Eu2+) with the extent of ceramization is explained by charge compensation model based on substitution defect mechanisms. Further,
the phenomenon of energy transfer from Eu2+ to Eu3+ ion by radiative trapping or re-absorption is evidenced which increases with the degree of ceramization. For the first time,
the reduction of Eu3+ to Eu2+ under normal air atmospheric condition has been observed in a BaF2 containing oxyfluoride glass-ceramics system.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1010-4
Authors
Kaushik Biswas, Glass Science and Technology Section, Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196, Raja S.C. Mullick Road, Kolkata, 700 032 India
Atul D. Sontakke, Glass Science and Technology Section, Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196, Raja S.C. Mullick Road, Kolkata, 700 032 India
R. Sen, Glass Science and Technology Section, Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196, Raja S.C. Mullick Road, Kolkata, 700 032 India
K. Annapurna, Glass Science and Technology Section, Central Glass and Ceramic Research Institute, Council of Scientific and Industrial Research, 196, Raja S.C. Mullick Road, Kolkata, 700 032 India
The photophysical properties of novel cyclic azacyanine derivatives have been investigated in acetonitrile, N-butyronitrile,
methanol, ethanol, DMF and water. Introduction of electron donating or accepting groups on the cyclic azacyanine has a direct
impact on the spectroscopic and photophysical properties. Irrespective of the nature of the substitution, azacyanine shows
a general solvent relaxation in accordance with Lippert-Mataga’s prediction; however, in protic solvent, specific interactions
are encountered. Fluorescence lifetime decay suggests a relaxation in the nanosecond time scale with monoexponential decay
in polar solvents and biexponential decay in non polar solvents. The fluorescence lifetime of azacyanines are found to be
longer than popular cy3 dyes. An electron donating substituent increases the fluorescence lifetime and influences the radiative
process, whereas an electron withdrawing group marginally increases the excited state lifetime but remarkably enhances the
radiative process. The fluorescence quantum yield of substituted cyclic azacyanine in water is noted to be at least five fold
higher than the popular cy3 dye.
Content Type Journal Article
Category Original Paper
Pages 1-11
DOI 10.1007/s10895-011-1006-0
Authors
Digambara Patra, Department of Chemistry, American University of Beirut, Beirut, Lebanon
Nagham N. Malaeb, Department of Chemistry, American University of Beirut, Beirut, Lebanon
Makhluf J. Haddadin, Department of Chemistry, American University of Beirut, Beirut, Lebanon
Mark J. Kurth, Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA
L-Cysteine capped CdTe nanoparticles (NPs) were synthesized in aqueous medium, and their application as fluorescence probes
in the determination of paracetamol was studied. The L-cysteine capped CdTe NPs were characterized by transmission electron
microscopy, X-ray diffraction spectrometry, spectrofluorometry, ultraviolet-visible and Fourier transform infrared spectrometry.
Based on the distinct fluorescence quenching of CdTe fluorescence probes in the presence of paracetamol, a simple, rapid and
specific method for paracetamol determination was presented. Under optimum conditions, the relative fluorescence intensity
of CdTe NPs was linearly proportional to paracetamol concentration from 1.0 × 10−8 mol/L to 1.6 × 10−7 mol/L with a detection limit of 4.2 × 10−9 mol/L. The proposed method was applied to detect paracetamol in commercial tablets with satisfactory results.
Content Type Journal Article
Category Original Paper
Pages 1-6
DOI 10.1007/s10895-011-0994-0
Authors
Li Li, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
Yaxiang Lu, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
Yaping Ding, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
Yu Cheng, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
Wanchen Xu, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
Fenfen Zhang, Department of Chemistry, Shanghai University, Shanghai, 200444 People’s Republic of China
A functionalized fluorescent conjugated polymer, tolylterpyridine poly(p-phenyleneethynylene-thienyleneethynylene (ttp-PPETE),
was designed and synthesized to detect trace amounts of toxic transition metal pollutants in ground water. Photophysical studies
in tetrahydrofuran (THF) successfully demonstrated this polymer as a selective and sensitive chemosensor for Ni2+ and Co2+ in aqueous solution. Solid state composites of these chemosensors have now been prepared which can be modified to provide
for inexpensive and portable field based chemical detection. A solid composite of ttp-PPETE, blended with poly (methyl methacrylate)
shows UV–vis absorption and fluorescence emission spectra which are red- shifted when compared to solution phase spectra,
suggesting an increase in conjugation in the solid state. An additional absorption peak, not present in solution, is also
observed in the solid state. The presence of this new peak provides evidence of interacting FCP chains in the solid state.
Concentration dependent experiments were done on the solid composite showing red-shifted emission peaks accompanied by a significant
reduction in the fluorescent quantum yield. These observations are consistent with the formation of aggregated polymer species
in the solid state. Intermolecular interactions of this type can be manipulated in the design of sensitive and selective solid
state fluorescent conjugated polymer sensors.
Content Type Journal Article
Category Original Paper
Pages 1-7
DOI 10.1007/s10895-011-0993-1
Authors
Sherryllene S. Pinnock, Chemistry Department, State University of New York at Binghamton, Binghamton, NY 13902, USA
Catherine N. Malele, Chemistry Department, State University of New York at Binghamton, Binghamton, NY 13902, USA
Justin Che, Chemistry Department, State University of New York at Binghamton, Binghamton, NY 13902, USA
Wayne E. Jones, Chemistry Department, State University of New York at Binghamton, Binghamton, NY 13902, USA
The steady-state absorption and fluorescence, as well as the time-resolved fluorescence properties of dimethoxycurcumin and
bis-dehydroxycurcumin dissolved in several solvents differing in polarity and H-bonding capability are presented. The singlet
oxygen generation efficiency of the two compounds relative to curcumin is estimated. The photodegradation quantum yield of
the former compound in acetonitrile and methanol is determined. The dimethoxycurcumin and bis-dehydroxycurcumin decay mechanisms
from the S1 state are discussed and compared with those of curcumin, dicinnamoylmethane and bis-demethoxycurcumin.
Content Type Journal Article
Category Original Paper
Pages 1-12
DOI 10.1007/s10895-011-0995-z
Authors
L. Nardo, Department of Physics and Mathematics, University of Insubria and C.N.I.S.M.-C.N.R., Via Valleggio, 11-22100 Como, Italy
A. Andreoni, Department of Physics and Mathematics, University of Insubria and C.N.I.S.M.-C.N.R., Via Valleggio, 11-22100 Como, Italy
M. Bondani, Institute for Photonics and Nanotechnology, C.N.R. (Consiglio Nazionale delle Ricerche), Via Valleggio, 11-22100 Como, Italy
M. Másson, Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Hagi, Hofsvallagata 53, IS-107 Reykjavik, Iceland
T. Haukvik, School of Pharmacy, University of Oslo, Blindern, P.O.Box 1068, 0316 Oslo, Norway
H. H. Tønnesen, School of Pharmacy, University of Oslo, Blindern, P.O.Box 1068, 0316 Oslo, Norway
In this paper, the organic ligands TTA and TAA are grafted onto the coupling agent to achieve the organic precursors (TTA-Si,
TAA-Si) as first ligand and the organic 4-(4-nitrostyryl)pyridine (Nspy) is synthesized as the second ligand. Both of them
are coordinated to the rare ions with the carbonyl group and nitrogen atom respectively. After the hydrolysis and copolycondensation
between the teraethoxysilane (TEOS) and the ternary rare earth organic complex via the sol–gel process, the chemical bonded
hybrid materials are constructed and characterized in detail. The obtained hybrid materials present superior thermal stabilities
and regular and homogenous square blocks microstructure. Among the hybrids Eu(TTA-Si)3Nspy shows a more strong characteristic emission and longer lifetime than the hybrids Eu(TAA-Si)3Nspy, while hybrids Tb(TAA-Si)3Nspy exhibits the stronger characteristic emission and longer lifetime than the hybrids Tb(TTA-Si)3Nspy. For Sm3+ hybrid materials, the photoluminescence of Sm(TAA-Si)3Nspy is too weak to find in the characteristic emission spectra, meanwhile, Sm(TTA-Si)3Nspy has the excellent luminescent intensity.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1008-y
Authors
Yan-Yan Li, Department of Chemistry, Tongji University, Siping Road 1239, Shanghai, 200092 China
Bing Yan, Department of Chemistry, Tongji University, Siping Road 1239, Shanghai, 200092 China
Lei Guo, Department of Chemistry, Tongji University, Siping Road 1239, Shanghai, 200092 China
A novel ligand with double sulfinyl groups, bis(benzylsulfinyl)methane, was synthesized by a new method and its two lanthanide
(III) complexes were synthesized and characterized by element analysis, molar conductivity, coordination titration analysis,
IR, TG-DSC, 1HNMR and UV spectra. The results indicated that the composition of these complexes was REL2.5(ClO4)3·3H2O (RE = Tb (III), Dy (III), L = C6H5CH2SOCH2SOCH2C6H5). The FT-IR results revealed that the perchlorate group was bonded with the lanthanide ion by the oxygen atoms, and the coordination
was bidentate. The fluorescent spectra illustrated that both the Tb (III) and Dy (III) complexes displayed characteristic
fluorescence in solid state, especially for the Tb (III) complex, the peak of 5D4 → 7 F5 of the Tb (III) ion in 544 nm was stronger than that of others. It indicated that the Tb (III) complex could emit purer green
fluorescence. By analysis fluorescence and phosphorescence spectra, it was found that the ligand had the advantage to absorb
energy and transfer it to the Tb (III) and Dy (III) ions. The phosphorescence spectra and fluorescence lifetimes of the complexes
were also measured.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-1001-5
Authors
Wen-Xian Li, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Ying-Jie Li, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Wen-Juan Chai, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Tie Ren, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Yu Liu, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Jing Zhang, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Bo-Yang Ao, College of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010021 People’s Republic of China
Two coumarin derivatives containing electron-transporting benzoxazolyl moiety, 7-(diethylamino)-3-(benzoxazol-2-yl)coumarin
(DABOC) and 3-(benzoxazol-2-yl)benzo[5,6]coumarin (BOBC), were synthesized and characterized. The photoluminescence and electroluminescence
of the compounds were investigated detailedly. The compounds exhibited strong blue-green emissions in both solution and solid
states, but the devices with DABOC as the emitting layer exhibited orange emission and maximum luminous efficiency of 2.8 cd/A
and maximum luminance of 8,800 cd/m2, and the devices with BOBC displayed orange-white emission and maximum luminous efficiency of 0.13 cd/A and maximum luminance
of 540 cd/m2.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0999-8
Authors
Yuling Zhao, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Tianzhi Yu, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Youzhi Wu, School of Materials Science & Engineering, Lanzhou University of Technology, Lanzhou, 730050 China
Hui Zhang, Key Laboratory of Opto-Electronic Technology and Intelligent Control (Lanzhou Jiaotong University), Ministry of Education, Lanzhou, 730070 China
Duowang Fan, Key Laboratory of Opto-Electronic Technology and Intelligent Control (Lanzhou Jiaotong University), Ministry of Education, Lanzhou, 730070 China
Zunwei Gan, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Liangliang Yang, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Xiaoqian Han, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Yumei Zhang, School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou, 730070 China
Single molecule detection and tracking provides at times the only possible method to observe the interactions of low numbers
of biomolecules, inlcuding DNA, receptors and signal mediating proteins in living systems. However, most existing imaging
methods do not enable both high sensitivity and non-invasive imaging of large specimens. In this study we report a new setup
for selective plane illumination microscopy (SPIM), which enables fast imaging and single molecule tracking with the resolution
of confocal microscopy and the optical penetration beyond 300 μm. We detect and report our instrumental figures of merit,
control values of fluorescence properties of single nano crystals in comparison to both standard widefield configurations,
and also values of nanocrystals in multicellular “fruiting bodies” of Dictyostelium, an excellent control as a model developmental system. In the Dictyostelium , we also report some of our first tracking of single nanocrystals with SPIM. The new SPIM setup represents a new technique,
which enables fast single molecule imaging and tracking in living systems.
Content Type Journal Article
Category Original Paper
Pages 1-11
DOI 10.1007/s10895-011-0966-4
Authors
Mike Friedrich, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
Revaz Nozadze, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
Sandra de Keijzer, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
Ralf Steinmeyer, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
Vladimir Ermolayev, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
Gregory S. Harms, Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Josef-Schneider-Str. 2, D15, 97080 Würzburg, Germany
A rapid, simple and sensitive spectrofluorimetric method for determination of trace amount of ofloxacin was developed. At
pH 5.1 the ofloxacin enhances the luminescence intensity of the Eu3+ ion in Eu3+- ofloxacin complex at λex = 365 nm. The produced luminescence intensity of Eu3+-ofloxacin complex was in proportional to the concentration of ofloxacin. The working range for the determination of ofloxacin
was 5.0 × 10-9–5.0 × 10-6 mol L-1 with lower detection limit (LOD) and quantitative detection limit (QDL) of 3 × 10-9 and 9 × 10-9 mol L-1, respectively. The enhancement mechanism of the luminescence intensity in the Eu3+-ofloxacin system has been also explained. The method revealed good selectivity for ofloxacin in the presence of coexisting
substances and used successfully for the assay of ofloxacin in pharmaceutical preparations and serum. A comparison with other
standard methods was also discussed.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0989-x
Authors
M. S. Attia, Chemistry Department, Ain Shams University, Cairo, Egypt
Amr A. Essawy, Chemistry Department, Fayoum University, Al Fayoum, Egypt
A. O. Youssef, Chemistry Department, Ain Shams University, Cairo, Egypt
Marwa S. Mostafa, Chemistry Department, Ain Shams University, Cairo, Egypt
A novel piezochromic fluorescent (PCF) compound with aggregation-induced emission (AIE) effect and morphology-alterable emission
property was developed. The amorphous and crystalline aggregates were obtained, and their spectroscopic properties and morphological
structures were reversibly and repeatedly exhibited upon pressing (fuming) or annealing. The piezochromic fluorescent nature
was generated through crystalline-amorphous phase transformation. It was proposed that AIE compounds existing a twisted propeller-shaped
conformation will exhibit PCF activity. The common relationship betweeen AIE and PCF established will guide researchers in
identifying and synthesizing more piezochromic fluorescent materials.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0990-4
Authors
Xie Zhou, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510275 China
Hai-yin Li, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Zhen-guo Chi, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Bing-jia Xu, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Xi-qi Zhang, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Yi Zhang, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Si-wei Liu, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
Jia-rui Xu, PCFM Lab, DSAPM Lab, KLGHEI of Environment and Energy Chemistry, FCM Institute, State Key Laboratory of Optoelectronic Materials and Technologies, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275 China
The use of fluorescent nanomaterials has gained great importance in the field of medical imaging. Many traditional imaging
technologies have been reported utilizing dyes in the past. These methods face drawbacks due to non-specific accumulation
and photobleaching of dyes. We studied the uptake and internalization of two different sized (30 nm and 100 nm) FITC labeled
silica nanoparticles in Human umbilical vein endothelial cell line. These nanomaterials show high biocompatability and are
highly photostable inside live cells for increased period of time in comparison to the dye alone. To our knowledge, we report
for the first time the use of 30 nm fluorescent silica nanoparticles as efficient endothelial tags along with the well studied
100 nm particles. We also have emphasized the good photostability of these materials in live cells.
Content Type Journal Article
Category Original Paper
Pages 1-12
DOI 10.1007/s10895-011-0991-3
Authors
Srivani Veeranarayanan, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Aby Cheruvathoor Poulose, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Sheikh Mohamed, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Athulya Aravind, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Yutaka Nagaoka, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Yasuhiko Yoshida, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
Toru Maekawa, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
D. Sakthi Kumar, Bio Nano Electronics Research Center Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Saitama 350-8585, Japan
A simple and sensitive method has been developed and validated for the determination of aliskiren (ALS) in its dosage forms
and spiked plasma. The method was based on the reaction of the drug with dansyl chloride in the presence of bicarbonate solution
of pH 10.5 to give a highly fluorescent derivative which was measured at 501 nm with excitition at 378 nm in dichloromethane.
Different experimental parameters affecting the development of the method and stability were carefully studied and optimized.
The calibration curves were linear over the concentration ranges of 100–700 and 50–150 ng/mL for standard solution and plasma,
respectively. The limits of detection were 27.52 ng/mL in standard solution, 4.91 ng/mL in plasma. The developed method was
successfully applied to the analysis the drug in the commercial tablets and spiked plasma samples. The mean recovery of ALS
from tablets and plasma was 100.10 and 97.81%, respectively. A proposal of the reaction pathway was presented.
Content Type Journal Article
Category Original Paper
Pages 1-8
DOI 10.1007/s10895-011-0988-y
Authors
Zeynep Aydoğmuş, Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazıt, Istanbul, Turkey
Ferhat Sarı, Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazıt, Istanbul, Turkey
Sevgi Tatar Ulu, Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, 34116 Beyazıt, Istanbul, Turkey
