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On this page considered biochemistry journals:
The Analyst - published by
The Royal Society of Chemistry, RSC -
... is the journal of choice for publishing urgent new work of the highest quality in analytical, bioanalytical and detection science.
Analytical and Bioanalytical Chemistry - published by
Springer -
ABC is a truly international journal with a mission to publish excellent research papers from all areas of analytical and bioanalytical science.
Analytical Chemistry - published by
The American Chemical Society, ACS -
... is a peer-reviewed research journal that explores the latest concepts in analytical measurements and the best new ways to increase accuracy, selectivity, sensitivity, and reproducibility.
Current research articles of the mentioned
journals:
Carlos Baleizão, Stefan Nagl, Michael Schäferling, Mário N. Berberan-Santos, and Otto S. Wolfbeis Web Release Date: Thu, 24 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac801034p
Tsoching Chen, Min-Jung Lee, Yeong Sang Kim, Sunmin Lee, Shivaani Kummar, Martin Gutierrez, Stephen M. Hewitt, Jane B. Trepel, and Ira W. Levin Web Release Date: Thu, 24 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800840y
Yang-Zhen Huang, Wen-Bin Du, Jian-Zhang Pan, Qun Fang
(Paper from Analyst)
Yang-Zhen Huang, Analyst, 2008, DOI: 10.1039/b807494a
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Abstract Polybrominated organic compounds are ubiquitous throughout the environment. This generic term comprises several classes of
brominated flame retardants (e.g., polybrominated diphenyl ethers, polybrominated biphenyls, hexabromocyclododecane, dibromopropyltribromophenyl
ether, 1,2-bis(2,4,6-tribromophenoxy)ethane) as well as a range of marine halogenated natural products (HNPs). Here we present
gas chromatography retention times and elution orders (on DB-5) of 122 polybrominated compounds that may be found in food
and environmental samples. Organobromine compounds in fish samples determined with gas chromatography interfaced to electron-capture
negative ion mass spectrometry (GC/ECNI-MS) are discussed. The environmental relevance and important mass spectrometric features
of the compounds are described as well. Our database aims to support the closer inspection and identification of peaks in
gas chromatograms and to initiate dedicated screening for less frequently studied organobromines in samples.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2277-4
Authors
Walter Vetter, Universität Hohenheim Institut für Lebensmittelchemie Garbenstr. 28 70593 Stuttgart Germany
Natalie Rosenfelder, Universität Hohenheim Institut für Lebensmittelchemie Garbenstr. 28 70593 Stuttgart Germany
Abstract Interest in biological studies on various cell types for many biomedical applications, from research to patient treatments,
is constantly increasing. The ability to discriminate (sort) and/or quantify distinct subpopulations of cells has become increasingly
important. For instance, not only detection but also the highest depletion of neoplastic cells from normal cells is an important
requisite in the autologous transplantation of lymphocytes for blood cancer treatments. In this work, gravitational field-flow
fractionation (GrFFF) is shown to be effective for sorting a heterogeneous mixture of human, living lymphocytes constituted
of neoplastic B cells from a Burkitt lymphoma cell line and healthy T and B lymphocytes from blood samples. GrFFF does not
require the use of fluorescent immunotags for sorting cells, and the sorted cells can be collected for their further characterization.
Flow cytometry was used to assess the viability of the cells collected, and to evaluate the cell fractionation achieved. A
low amount of neoplastic B lymphocytes (less than 2%) was found in a specific fraction obtained by GrFFF. The high depletion
from neoplastic cells (more than 98%) was confirmed by a clonogenicity test.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2271-x
Authors
Barbara Roda, University of Bologna Department of Chemistry “G. Ciamician” Via Selmi 2 40126 Bologna Italy
Pierluigi Reschiglian, University of Bologna Department of Chemistry “G. Ciamician” Via Selmi 2 40126 Bologna Italy
Andrea Zattoni, University of Bologna Department of Chemistry “G. Ciamician” Via Selmi 2 40126 Bologna Italy
Pier Luigi Tazzari, S. Orsola-Malpighi Hospital Service of Transfusion Medicine 40138 Bologna Italy
Marina Buzzi, S. Orsola-Malpighi Hospital Service of Transfusion Medicine 40138 Bologna Italy
Francesca Ricci, S. Orsola-Malpighi Hospital Service of Transfusion Medicine 40138 Bologna Italy
Andrea Bontadini, S. Orsola-Malpighi Hospital Service of Transfusion Medicine 40138 Bologna Italy
Abstract The external sixteenth century wall paintings of St. Dumitru’s Church in Suceava (Romania) are suffering visually from deterioration.
Fragments of these paintings spallated from the external wall have been studied by micro-Raman microscopy in order to elucidate
possible causes of this process. Calcite and α-quartz are the components of the substratum indicating that the artists used
the Roman fresco technique comprising a limewash putty. No organic binders have been detected in the substrate or pigment
application. Amorphous carbon and goethite, α-FeOOH, have been identified in areas containing residues of grey and yellow
pigments, respectively. Small amounts of gypsum have been detected in the grey areas which we attribute to special attention
being given to surface preparation and pigment application in these areas. An abundance of sodium nitrate, nitratine, microcrystals
have been observed on the surfaces of many fragments which suggests that a biodeterioration process originating from guano
deposits could have been operating in these frescoes.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2262-y
Authors
A. Hernanz, Universidad Nacional de Educación a Distancia (UNED) Departamento de Ciencias y Técnicas Fisicoquímicas, Facultad de Ciencias Senda del Rey 9 28040 Madrid Spain
I. Bratu, National Institute for R&D of Isotopic and Molecular Technologies P.O. Box 700 400293 Cluj-Napoca 5 Romania
O. F. Marutoiu, Babes-Bolyai University Faculty of Chemistry and Chemical Engineering 11 A. Janos st. 400028 Cluj-Napoca Romania
C. Marutoiu, Babes-Bolyai University Faculty of Orthodox Theology 18 A. Iancu st. 400084 Cluj-Napoca Romania
J. M. Gavira-Vallejo, Universidad Nacional de Educación a Distancia (UNED) Departamento de Ciencias y Técnicas Fisicoquímicas, Facultad de Ciencias Senda del Rey 9 28040 Madrid Spain
H. G. M. Edwards, University of Bradford Division of Chemical and Forensic Sciences, School of Life Sciences Bradford BD7 1DP UK
Graham T. Eyres, Sylvia Urban, Paul D. Morrison, Jean-Pierre Dufour, and Philip J. Marriott Web Release Date: Wed, 23 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac8007847
Timothy R. Kline, Matthew K. Runyon, Mohammad Pothiawala, and Rustem F. Ismagilov Web Release Date: Wed, 23 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800485q
Huan Li, Choiwan Lau, Jianzhong Lu
(Paper from Analyst)
Huan Li, Analyst, 2008, DOI: 10.1039/b804096f
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Abstract A variety of bulk polymers for the selective separation of chloramphenicol were synthesised from 2-vinylpyridine, diethylaminoethyl
methacrylate or methacrylic acid monomers. Chromatographic evaluation indicated that chloramphenicol was retained under nonpolar
elution conditions (k = 58.65) through selective hydrogen bonding and ionic interactions. The retention of chloramphenicol under aqueous elution
conditions (k > 100) results from nonselective hydrophobic interactions. Under nonpolar elution conditions, the functional monomer employed
imparted a significant influence on the recognition properties of the corresponding polymer. After solid-phase extraction
using a molecularly imprinted polymer as sorbent and either an organic or aqueous washing solvent, nearly 100% recovery from
the chloramphenicol standard solution was achieved, and nearly 90% recovery could be attained from spiked honey samples. The
molecularly imprinted polymer was well suited to suppress matrix effects, and provided optimal preconcentration of the target
molecule (chloramphenicol) prior to chromatographic analysis.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2269-4
Authors
Christina Schirmer, Max Rubner Institute, Federal Research Institute of Nutrition and Food Department of Safety and Quality of Milk and Fish Products Hermann-Weigmann-Str. 1 24103 Kiel Germany
Hans Meisel, Max Rubner Institute, Federal Research Institute of Nutrition and Food Department of Safety and Quality of Milk and Fish Products Hermann-Weigmann-Str. 1 24103 Kiel Germany
Abstract Prohormones such as dehydroepiandrosterone (DHEA) are steroid precursors that do not show hormonal activity by themselves.
Abuse of these prohormones in cattle fattening is hard to prove because of strong in vivo metabolism and the difficulty to
detect metabolites which are not significantly above endogenous levels. The aim of the present work was to develop an in vitro
assay capable of detecting the indirect hormonal activity of prohormones that might be present in feed supplements and injection
preparations. Sample extracts were incubated with a bovine liver S9 fraction in order to mimic the in vivo metabolic activation.
Subsequently incubated extracts were exposed to a highly androgen-specific yeast bioassay to detect hormonal activity. Metabolic
activation of DHEA, 4-androstene-3,17-dione (4-adione) and 5-androstene-3,17-diol (5-adiol) resulted in an increased androgenic
activity caused by the formation of the active androgen 17β-testosterone (17β-T), as shown by ultra-performance liquid chromatography
and time-of-flight mass spectrometry with accurate mass measurement. The developed in vitro system successfully mimics the
hydroxysteroid dehydrogenase (HSD)- and cytochrome P450-mediated in vivo metabolic transitions, thus allowing assessment of
both bioactivity and chemical identification without the use of animal experiments. Screening of unknown supplement samples
claimed to contain DHEA resulted in successful bioactivation and positive screening results according to the androgen yeast
biosensor.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2275-6
Authors
J. C. W. Rijk, RIKILT - Institute of Food Safety, Wageningen UR P.O. Box 230 6700 AE Wageningen The Netherlands
T. F. H. Bovee, RIKILT - Institute of Food Safety, Wageningen UR P.O. Box 230 6700 AE Wageningen The Netherlands
M. J. Groot, RIKILT - Institute of Food Safety, Wageningen UR P.O. Box 230 6700 AE Wageningen The Netherlands
A. A. C. M. Peijnenburg, RIKILT - Institute of Food Safety, Wageningen UR P.O. Box 230 6700 AE Wageningen The Netherlands
M. W. F. Nielen, RIKILT - Institute of Food Safety, Wageningen UR P.O. Box 230 6700 AE Wageningen The Netherlands
J. Throck Watson and O. David Sparkman: Introduction to mass spectrometry. Instrumentation, applications, and strategies for data interpretation, 4th ed.
Content Type Journal Article
Category Books and Software in Review
DOI 10.1007/s00216-008-2246-y
Authors
Jürgen H. Gross, University of Heidelberg Institute of Organic Chemistry Im Neuenheimer Feld 270 69120 Heidelberg Germany
Abstract A method was developed for the determination of phosphoamino acids by capillary zone electrophoresis–laser-induced fluorescence
detection (argon ion laser, excitation at 488 nm and emission at 520 nm) using derivatization with N-hydroxysuccinimidyl fluorescein-O-acetate (SIFA). Different variables affecting the derivatization (SIFA concentration, derivatization pH, reaction temperature
and reaction time) and the separation (type, pH and concentration of buffer, applied voltage and injection mode) were investigated
in detail. The optimized separation conditions were 40 mM boric acid buffer (pH 9.2) for background electrolyte, 25 kV for
the separation voltage, 25 °C for the capillary temperature and 5 s at 0.5 psi for the sample injection. Under the optimal
conditions, the SIFA-labeled phosphoamino acids were fully separated within 7 min. The detection limits ranged from 0.1 to
0.3 nM, which are the lowest values reported for capillary electrophoresis (CE) methods. The proposed methodology allowed
the rapid, sensitive and selective determination of phosphoamino acids in hen egg yolk phosvitin by the standard addition
method. The recovery of these compounds in real sample was 94.0–103.5%. The developed method surpasses previously published
CE methods in terms of detection limit, separation time, stability and simplicity of the electrophoretic procedure.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2245-z
Authors
Ying-Hua Deng, Wuhan University Department of Chemistry Wuhan 430072 China
Hua-Shan Zhang, Wuhan University Department of Chemistry Wuhan 430072 China
Hong Wang, Wuhan University Department of Chemistry Wuhan 430072 China
Caroline N. Jones, Ji Youn Lee, James Zhu, Gulnaz Stybayeva, Erlan Ramanculov, Mark A. Zern, and Alexander Revzin Web Release Date: Tue, 22 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac8007626
Nicolai Bache, Kasper D. Rand, Peter Roepstorff, and Thomas J. D. Jørgensen Web Release Date: Tue, 22 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800902a
Haode Chen, Xiaolei Zuo, Shao Su, Zhuzhao Tang, Aibo Wu, Shiping Song, Dabing Zhang, Chunhai Fan
(Paper from Analyst)
Haode Chen, Analyst, 2008, DOI: 10.1039/b805334k
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The content of this RSS Feed (c) The Royal Society of Chemistry
Zhuomin Zhang, Dapeng Wang, Lan Zhang, Min Du, Guonan Chen
(Paper from Analyst)
Zhuomin Zhang, Analyst, 2008, DOI: 10.1039/b802679c
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry
Abstract As a continuation of our work on boronic acid lectin affinity chromatography (BLAC), in this paper we introduce an automated
affinity micropartitioning approach using combined boronic acid and concanavalin A (BLAC/Con A) resin-filled micropipette
tips to isolate and enrich human serum glycoproteins. The N-linked oligosaccharides of the partitioned glycoproteins were removed by PNGase F enzyme digestion, followed by 8-aminopyrene-1,3,6-trisulfonic
acid labeling. Capillary gel electrophoresis with blue LED-induced fluorescence detection was applied in a multiplexed format
for comparative glycan profiling. The efficiency of BLAC affinity micropartitioning was compared with that of the individual
lectin and pseudolectin affinity enrichment. Finally, we report on our findings in glycosylation differences in human serum
samples from healthy and prostate cancer patients by applying BLAC/Con A micropipette tip-based enrichment and comparative
multicapillary gel electrophoresis analysis of the released and labeled glycans.
Figure Fluorophore labeling and purification in BLAC–mCE based glycosylation profiling
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2257-8
Authors
Alex Monzo, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
Marcell Olajos, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
Lorenzo De Benedictis, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
Zuly Rivera, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
Guenther K. Bonn, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
András Guttman, University of Innsbruck Horváth Laboratory of Bioseparation Sciences, Institute of Analytical Chemistry and Radiochemistry Innrain 66 Innsbruck Austria
Abstract Square-wave anodic-stripping voltammetry (SWASV) was set up and optimized for simultaneous determination of cadmium, lead,
and copper in siliceous spicules of marine sponges, directly in the hydrofluoric acid solution (∼0.55 mol L−1 HF, pH ∼1.9). A thin mercury-film electrode (TMFE) plated on to an HF-resistant epoxy-impregnated graphite rotating-disc
support was used. The optimum experimental conditions, evaluated also in terms of the signal-to-noise ratio, were as follows:
deposition potential −1100 mV vs. Ag/AgCl, KCl 3 mol L−1, deposition time 3–10 min, electrode rotation 3000 rpm, SW scan from −1100 mV to +100 mV, SW pulse amplitude 25 mV, frequency
100 Hz, ΔEstep 8 mV, tstep 100 ms, twait 60 ms, tdelay 2 ms, tmeas 3 ms. Under these conditions the metal peak potentials were Cd −654 ± 1 mV, Pb −458 ± 1 mV, Cu −198 ± 1 mV. The electrochemical
behaviour was reversible for Pb, quasi-reversible for Cd, and kinetically controlled (possibly following chemical reaction)
for Cu. The linearity of the response with concentration was verified up to ∼4 μg L−1 for Cd and Pb and ∼20 μg L−1 for Cu. The detection limits were 5.8 ng L−1, 3.6 ng L−1, and 4.3 ng L−1 for Cd, Pb, and Cu, respectively, with td = 5 min. The method was applied for determination of the metals in spicules of two specimens of marine sponges (Demosponges)
from the Portofino natural reserve (Ligurian Sea, Italy, Petrosia ficiformis) and Terra Nova Bay (Ross Sea, Antarctica, Sphaerotylus antarcticus). The metal contents varied from tens of ng g−1 to ∼1 μg g−1, depending on the metal considered and with significant differences between the two sponge species.
Marine sponges Petrosia ficiformis and Sphaerotylus antarcticus analysed by voltammetry (Photos Carlo Cerrano)
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2239-x
Authors
C. Truzzi, Polytechnic University of Marche – Ancona Department of Marine Science Via Brecce Bianche 60131 Ancona Italy
A. Annibaldi, Polytechnic University of Marche – Ancona Department of Marine Science Via Brecce Bianche 60131 Ancona Italy
S. Illuminati, Polytechnic University of Marche – Ancona Department of Marine Science Via Brecce Bianche 60131 Ancona Italy
E. Bassotti, Polytechnic University of Marche – Ancona Department of Marine Science Via Brecce Bianche 60131 Ancona Italy
G. Scarponi, Polytechnic University of Marche – Ancona Department of Marine Science Via Brecce Bianche 60131 Ancona Italy
Abstract The unique properties of mesoporous silica materials (MPs) have attracted substantial interest for use as enzyme-immobilization
matrices. These features include high surface area, chemical, thermal, and mechanical stability, highly uniform pore distribution
and tunable pore size, high adsorption capacity, and an ordered porous network for free diffusion of substrates and reaction
products. Research demonstrated that enzymes encapsulated or entrapped in MPs retain their biocatalytic activity and are more
stable than enzymes in solution. This review discusses recent advances in the study and use of mesoporous silica for enzyme
immobilization and application in biosensor technology. Different types of MPs, their morphological and structural characteristics,
and strategies used for their functionalization with enzymes are discussed. Finally, prospective and potential benefits of
these materials for bioanalytical applications and biosensor technology are also presented.
Figure Enzyme-functionalized mesoporous silica fibers and their integration in a biosensor design. The immobilization process takes
place essentially in the silica micropores.
Content Type Journal Article
Category Review
DOI 10.1007/s00216-008-2250-2
Authors
Cristina Ispas, Clarkson University Department of Chemistry and Biomolecular Science Potsdam NY 13699–5810 USA
Igor Sokolov, Clarkson University Department of Physics Potsdam NY 13699–5810 USA
Silvana Andreescu, Clarkson University Department of Chemistry and Biomolecular Science Potsdam NY 13699–5810 USA
Abstract Vascular basement membrane remodeling is involved in tumor angiogenesis to enable tumor invasion and growth. FT-IR spectral
imaging was used to determine changes in tumor blood vessels to reveal protein secondary structure in Rag-gamma immuno-deficient
mice sacrificed 14 and 21 days after subcutaneous glioma implantation. For the oldest blood capillaries (diameter >20 microns),
tumor growth induced a decrease in triple-helix content (1638 cm−1; −7.3%; P < 0.05) and an increase in beta turns (1666 and 1615 cm−1; +4%; P < 0.01). These protein-structure alterations, mainly from type IV collagen, reflected the high angiogenic stress of growing
tumors. We propose to use these molecular markers of vascular basement membrane protein alterations for gradation of solid
tumors by FT-IR spectral imaging.
Figure From spectral curve fitting to tumor signature by blood capillaries FT-IR imaging was used to analyze vascular basement membrane
protein contents changes along tumor growth. Changes in protein secondary structure (decrease in triple helix and increase
in β-turns contents) revealed the high angiogenic stress of growing tumors. These structural changes might be used as molecular
markers for a functional FT-IR imaging of tumor growth. FT-IR imaging of a tumor blood capillary. Spectral curve-fitting of
pixels corresponding to vascular basement membrane of the capillary is used to determine secondary structure of protein contents.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2230-6
Authors
Katia Wehbe, Université de Bordeaux, CNRS UMR 5084, CNAB 146 Rue Léo Saignat 33076 Bordeaux-Cedex France
Raphael Pinneau, Université Bordeaux 1, INSERM U920 Avenue des Facultés 33405 Talence-Cedex France
Michel Moenner, Université Bordeaux 1, INSERM U920 Avenue des Facultés 33405 Talence-Cedex France
Gérard Déléris, Université de Bordeaux, CNRS UMR 5084, CNAB 146 Rue Léo Saignat 33076 Bordeaux-Cedex France
Cyril Petibois, Université de Bordeaux, CNRS UMR 5084, CNAB 146 Rue Léo Saignat 33076 Bordeaux-Cedex France
Abstract This paper presents both biological and potentiometric evaluations of the cell toxicity of a widely used ionic liquid, 1-butyl-3-methylimidazolium
tetrafluoroborate ([bmim]BF4), to Chinese hamster lung fibroblast cells (V79 cell line). The innovative potentiometric study takes advantage of the unique
properties of conductive polymer polypyrrole (PPY) for the potentiometric evaluation of cell toxicity of [bmim]BF4 to the V79 cells in a real-time, noninvasive and high-throughput manner. The conductive polymer PPY provides a controlled
microenvironment that allows the quantitative release of the anions of the ionic liquids into the cells being monitored in
real time and noninvasively. Parallel biological assay results showed that V79 cells exposed to [bmim]BF4 usually grew in clusters, and that many small vacuoles could be seen in the cytoplasm. At the 24th hour after the V79 cells
had been exposed to the ionic liquid (IL), the half inhibition concentration (EC50) of [bmim]BF4 was around 5 mM. From a cell cycle study performed using a FACScan flow cytometer, it was found that the V79 cells could
be partially locked to the G1 phase by [bmim]BF4, which extended the doubling time for cell growth. Comparing with the EC50 values of cadmium chloride and mercury chloride, [bmim]BF4 is not very toxic, but it may have a long-term toxic effect on mammalian cells. Compared to traditional biological in vitro
assays, the use of a conductive polymer substrate in combination with a potentiometric sensor array is much more sensitive,
faster, and enables a simpler evaluation of chemical cell toxicity. Additionally, it simplifies the study of the reversibility
of cell toxicity, i.e., cell recovery, because there is no need to refresh the culture medium since a finite amount of chemicals
can be doped and released. We found that the cytotoxicity of [bmim]BF4 at a concentration of less than 6 mM was reversible for the V79 cell line, because cell morphology and proliferation rate
returned to normal after the removal of the IL from the culture medium. This finding suggests that the IL [bmim]BF4 could be used as a tool to control mammalian cell proliferation rate.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2263-x
Authors
Weilian Qiu, Oakland University Chemistry Department Rochester MI 48309 USA
Xiangqun Zeng, Oakland University Chemistry Department Rochester MI 48309 USA
Nimer Wehbe, Andreas Heile, Heinrich F. Arlinghaus, Patrick Bertrand, and Arnaud Delcorte Web Release Date: Thu, 17 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800568y
Xuan Liu, Lingxiao Cheng, Jianping Lei, Huangxian Ju
(Communication from Analyst)
Xuan Liu, Analyst, 2008, DOI: 10.1039/b807183g
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The content of this RSS Feed (c) The Royal Society of Chemistry
Abstract The biotransformation capacity – of the cytochrome P450 (CYP) system for example – is lower but inducibility is more pronounced
in neonates than in adults. On the other hand, both enzyme activities and inducibility decline with senescence. Precision-cut
rat liver slices are widely used as an in vitro tool for the examination of drug toxicity, xenobiotic metabolism or enzyme
induction. The aim of the present study was to assess whether age-related changes in CYP activities and induction observed
in vivo are also mirrored in vitro in liver slices. For this purpose, different CYP model reactions were measured in precision-cut
liver slices from one-day-old, 40-day-old and one-year-old rats after in vitro exposure to various inducers. Similar to the
in vivo situation, basal CYP activities were distinctly lower and inducibility was much more pronounced in liver slices from
neonatal than in those from adult animals. Also, enzyme activities were mostly somewhat lower in liver slices from aged rats
compared to those from 40-day-old rats. However, CYP inducibility was less pronounced than with younger animals too. Thus,
precision-cut rat liver slices are a suitable in vitro tool for investigating age-related changes in CYP activities and induction
as well as developmental differences in drug metabolism and toxicity.
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2253-z
Authors
Amelie Lupp, Friedrich Schiller University Jena Institute of Pharmacology and Toxicology Nonnenplan 4 07743 Jena Germany
Reinhild Glöckner, Friedrich Schiller University Jena Institute of Pharmacology and Toxicology Nonnenplan 4 07743 Jena Germany
Joachim Etzrodt, Friedrich Schiller University Jena Institute of Pharmacology and Toxicology Nonnenplan 4 07743 Jena Germany
Dieter Müller, Friedrich Schiller University Jena Institute of Pharmacology and Toxicology Nonnenplan 4 07743 Jena Germany
Jacquie T. Keer and Lyndsey Birch (Eds.): Essentials of nucleic acid analysis. A robust approach
Content Type Journal Article
Category Books and Software in Review
DOI 10.1007/s00216-008-2268-5
Authors
Sapna K. Deo, Indiana University Purdue University Indianapolis Department of Chemistry & Chemical Biology LD326 Blackford Street Indianapolis IN 46202 USA
Joseph R. Lakowicz, Krishanu Ray, Mustafa Chowdhury, Henryk Szmacinski, Yi Fu, Jian Zhang, Kazimierz Nowaczyk
(Critical Review from Analyst)
Joseph R. Lakowicz, Analyst, 2008, DOI: 10.1039/b802918k
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The content of this RSS Feed (c) The Royal Society of Chemistry
Abstract The concept used to realize the modulation of molecular recognition in a molecularly imprinted polymer is presented. Creating
a thermal phase transition within the binding framework, the imprinted material was prepared using Boc-phenylalanine as the
template and pNIPAM as the sensitive unit. The results indicate that such a transition causes a clear modulation in the recognition
behavior of the prepared polymer which depends on the operation temperature. At a relatively low temperature, the prepared
polymer acts like a traditionally imprinted one, showing a highly specific recognition for the imprint species. However, the
prepared polymer does not present any notable resolution at 40 °C. This recognition behavior is comparable to a process that
can be switched on and off, thus making modulated recognition feasible.
Figure A unique molecularly imprinted polymer capable of showing on/off-switchable behavior was prepared. At a relatively low temperature,
the prepared polymer acts like a traditionally imprinted one, showing a highly specific recognition for the imprint species.
However, the prepared polymer presents no notable resolution at 40 °C
Content Type Journal Article
Category Original Paper
DOI 10.1007/s00216-008-2254-y
Authors
Songjun Li, Central China Normal University Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Wuhan 430079 China
Xing Huang, Central China Normal University Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Wuhan 430079 China
Mingxia Zheng, Central China Normal University Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Wuhan 430079 China
Wuke Li, Central China Normal University Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry Wuhan 430079 China
Bryan J. McCullough, Jason Kalapothakis, Hayden Eastwood, Paul Kemper, Derek MacMillan, Karen Taylor, Julia Dorin, and Perdita E. Barran Web Release Date: Tue, 15 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800651b
Jonathan R. E. Thabano, Michael C. Breadmore, Joseph P. Hutchinson, Cameron Johns, Paul R. Haddad
(Paper from Analyst)
Jonathan R. E. Thabano, Analyst, 2008, DOI: 10.1039/b804805c
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The content of this RSS Feed (c) The Royal Society of Chemistry
Thomas A. Schmitz, Gerardo Gamez, Patrick D. Setz, Liang Zhu, and Renato Zenobi Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac8005044
Zhuangzhi Wang, Thomas Wilkop, Jong Ho Han, Yi Dong, Matthew J. Linman, and Quan Cheng Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800845w
Dawid R. Zalewski, Dietrich Kohlheyer, Stefan Schlautmann, and Han J. G. E. Gardeniers Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800567n
Gaurav Saini, Li Yang, Milton L. Lee, Andrew Dadson, Michael A. Vail, and Matthew R. Linford Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800209c
Jongcheol Seo, Min-Soo Suh, T. Daniel Thangadurai, Jinhee Kim, Young Ho Rhee, Hye-Joo Yoon, and Seung Koo Shin Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Accelerated Article) DOI: 10.1021/ac801007y
Alexander A. Marchione, Paul J. Fagan, Eric J. Till, Robert L. Waterland, and Concetta LaMarca Web Release Date: Sat, 12 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800883t
Robert. J. Meier, Mark-Steven Steiner, Axel Duerkop, and Otto S. Wolfbeis Web Release Date: Fri, 11 Jul 2008 00:00:00 EDT (Article) DOI: 10.1021/ac800581v
Nicoletta Nicolaou, Royston Goodacre
(Paper from Analyst)
Nicoletta Nicolaou, Analyst, 2008, DOI: 10.1039/b804439b
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Zhuo Du, Yong-Liang Yu, Xiao-Rui Yan, Jian-Hua Wang
(Paper from Analyst)
Zhuo Du, Analyst, 2008, DOI: 10.1039/b804347g
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